高树峰 1王心涛 1游龙贵 1黄芸 1潘其彬 1卢晓华2
作者信息
- 1. 赣州市人民医院耳鼻咽喉头颈外科,江西赣州 341000
- 2. 赣州市第五人民医院五官科,江西赣州 341000
- 折叠
摘要
Abstract
Objective To investigate the effects of baicalein on apoptosis and immune escape of laryngeal carcinoma cells and its mechanism.Methods Human laryngeal carcinoma TU686 cells were randomly divided into control group(routine culture),baicalein group(50 μmol·L-1 baicalein),Vector group(transfected with Vector+50 μmol·L-1 baicalein)and CD47 group[transfected with differentiation cluster 47(CD47)+50 μmol·L-1 baicalein].Cell proliferation and apoptosis were detected by 5-acetylidene-2'-deoxyuridine(Edu)method and Annexin V-FITC/PI double staining method.The expression of proliferative and apoptotic proteins were detected by Western blot.Human peripheral lymphocytes were co-cultured with cells in each group to detect the killing rate of TU686 cells.The phagocytosis rate of TU686 cells was observed.Results The Edu positive cell rates in control group,baicalein group,Vector group and CD47 group were(36.24±2.74)％,(13.33±1.93)％,(14.22±1.48)％and(27.80±2.74)％,respectively;the apoptosis rates were(3.94±0.40)％,(28.40±1.76)％,(26.60±1.41)％and(12.33±0.84)％,respectively;the relative expressions of CD47 protein were 1.00±0.11,0.42±0.07,0.44±0.06 and 1.21±0.12,respectively;the relative expression levels of CyclinD1 protein were 0.86±0.08,0.37±0.05,0.36±0.04 and 0.74±0.08,respectively;the relative expression levels of cleaved cysteine aspartate proteinase-7(Cl-Caspase-7)protein were 0.35±0.04,0.99±0.12,1.00±0.11 and 0.50±0.06,respectively;the killing rates of human peripheral lymphocytes to TU686 cells(1∶1 ratio)were(10.61±1.19)％,(32.02±2.27)％,(31.86±2.06)％and(14.39±1.07)％,respectively;the phagocytosis rates were(6.35±0.71)％,(11.99±1.64)％,(12.03±0.76)％and(7.06±0.57)％,respectively.The above indicators,baicalein group compared with the control group,Vector group compared with CD47 group,the differences were statistically significant(P＜0.05,P＜0.01,P＜0.001).Conclusion Baicalein may induce the apoptosis of laryngeal carcinoma cells and inhibit the immune escape of laryngeal carcinoma cells by inhibiting the expression of CD47,thus slowing down the progression of laryngeal carcinoma.关键词
黄芩素/喉癌/细胞凋亡/免疫逃逸/分化簇47Key words
baicalein/laryngeal cancer/cell apoptosis/immune escape/differentiation cluster 47分类
医药卫生
