摘要
Abstract
Objective Study on the long non-coding RNA(lncRNA)LINC00922 regulating secreted frizzled related protein 1(SFRP1)to inhibit the malignant progression of human oral mucosal fibroblasts(hOMF)induced by arecoline.Methods Cells were divided into blank control(NC)group(hOMF cells were not treated in any way),model group(60 mg·L-1 arecoline treatment),oe-LINC02147 group(60 mg·L-1 arecoline treatment,transfected with overexpression of LINC02147 vector),si-SFRP1 group(60 mg·L-1 arecoline treatment,transfected with overexpression of LINC02147 vector,transfected with SFRP1 inhibitor vector).Real-time fluorescence quantitative polymerase chain reaction was used to detect the relative expression levels of SFRP1 mRNA in each group of cells;immunofluorescence was used to detect the positive expression of alpha-smooth muscle actin(α-SMA)and vimentin in each group of cells;5-ethynyl-2'-deoxyuridine was used to detect the proliferation rate of each group of cells;dichlorodihydrofluorescein diacetate fluorescence probe method was used to detect the content of reactive oxygen species(ROS)in each group of cells.Results The relative expression levels of SERP1 mRNA in model group and oe-LINC02147 group were 1.00±0.08 and 3.75±0.59,respectively;the cell proliferation rates of NC group,model group,oe-LINC02147 group and si-SFRP1 group were(43.61±6.97)％,(12.98±4.27)％,(38.66±5.12)％and(15.73±5.42)％,respectively;ROS content were(9.42±1.73)％,(36.58±6.16)％,(12.89±2.08)％and(33.45±5.76)％,respectively;α-SMA positive expression were 1.00±0.13,5.02±1.46,2.36±0.48 and 4.37±0.97,respectively;the positive expressions of vimentin were 1.00±0.15,4.63±1.51,1.98±0.85 and 3.92±1.36,respectively.The above indexes in the model group were compared with the NC group,the above indexes in the oe-LINC02147 group were compared with the model group,and the above indexes in the si-SFRP1 group were compared with the oe-LINC02147 group,and the differences were statistically significant(all P＜0.001).Conclusion LINC02147 may inhibit the malignant progression of arecoline-induced human oral mucosa fibroblasts by regulating SFRP1.关键词
长链非编码RNA/槟榔碱/分泌型卷曲相关蛋白1/人口腔黏膜成纤维细胞Key words
long non-coding RNA/arecoline/secreted frizzled-related protein 1/human oral mucosal fibroblasts cell分类
医药卫生
