中国临床药理学杂志2025，Vol.41Issue(3)：345-349,5.DOI:10.13699/j.cnki.1001-6821.2025.03.010
circRNA_0044556在紫杉醇耐药三阴性乳腺癌中的作用研究
Effects of circRNA_044556 on paclitaxel resistance in triple-negative breast cancer
摘要
Abstract
Objective To investigate the effect of circRNA_0044556 on the resistance of triple-negative breast cancer(TNBC)to paclitaxel(PTX)and the mechanism of drug resistance.Methods PTX resistant TNBC cells(MDA-MB-231/PTX)were obtained by exposing the human TNBC cell(MDA-MB-231)to increasing concentrations of PTX.MDA-MB-231 and MDA-MB-231/PTX cells in logarithmic growth phase were treated with circRNA_0044556 short hairpin RNA(sh-circRNA_0044556)and negative control(sh-NC),respectively.The groups were finally divided into MDA-MB-231-NC,MDA-MB-231-circRNA_0044556,MDA-MB-231/PTX-NC and MDA-MB-231/PTX-circRNA_0044556 groups.The expression level of circRNA_0044556 in cells was determined by real-time quantitative polymerase chain reaction(RT-qPCR);methyl thiazolyl tetrazolium assay and colony formation assay were used to observe cell proliferation;flow cytometry was used to observe cell apoptosisand;Western blot was used to observe the expression of cell proliferation and apoptosis-related proteins.Results The relative expression levels of circRNA_0044556 in MDA-MB-231/PTX,MDA-MB-231 and MCF-10A cells were 6.08±0.59,3.36±0.11 and 1.00±0.02,respectively,and the differences were statistically significant(all P＜0.001).Proliferation rates of MDA-MB-231-NC and MDA-MB-231-circRNA_0044556 groups were(100.00±0.84)％and(54.38±1.20)％;the apoptosis rates were(2.41±0.34)％and(21.65±0.29)％,respectively,and the differences were statistically significant(all P＜0.001).The proliferation rates of MDA-MB-231/PTX-NC and MDA-MB-231/PTX-circRNA_0044556 groups were(112.29±1.19)％and(61.33±1.24)％;the apoptosis rates were(2.08±0.07)％and(14.38±0.16)％,respectively,the differences were all statistically significant(all P＜0.001).The relative protein expression level of Ki-67 in MDA-MB-231-NC and MDA-MB-231-circRNA_0044556 groups were 1.00±0.02 and 0.67±0.02;the relative protein expression of Bcl-2-associated X protein(Bax)were 1.00±0.02 and 1.46±0.03;and the differences were statistically significant(all P＜0.05).The relative relative protein expression levels of Ki-67 in MDA-MB-231/PTX-NC group and MDA-MB-231/PTX-circRNA_0044556 group were 1.00±0.03 and 0.81±0.01,respectively;the relative expression levels of Bax protein were 1.00±0.02 and 1.55±0.02,respectively;the relative expression levels of Bcl-2 protein were 1.00±0.03 and 0.79±0.01,respectively.The above indexes in MDA-MB-231/PTX-NC group were significantly different from those in MDA-MB-231/PTX-circRNA_0044556 group(all P＜0.05).Conclusions Knocking down the expression of circRNA_0044556 can inhibit the malignant progression and PTX resistance of TNBC cells.关键词
三阴性乳腺癌/环状RNA_0044556/增殖/凋亡/紫杉醇抵抗Key words
triple-negative breast cancer/circRNA_0044556/proliferation/apoptosis/resistance to paclitaxel分类
医药卫生引用本文复制引用
陈晶晶,石鹏,王宏,常猛,蒋楠,马杰..circRNA_0044556在紫杉醇耐药三阴性乳腺癌中的作用研究[J].中国临床药理学杂志,2025,41(3):345-349,5.基金项目
河北省医学科学研究课题计划基金资助项目(20211662) （20211662）
