中国临床药理学杂志2025，Vol.41Issue(3)：350-354,5.DOI:10.13699/j.cnki.1001-6821.2025.03.011
Siglec-15调节STAT1/STAT3通路促进肝癌细胞增殖和迁移的研究
Siglec-15 regulates the STAT1/STAT3 pathway to promote the proliferation and migration of hepatocellular carcinoma cells
摘要
Abstract
Objective Probing whether salivary acid-binding immunoglobulin-like agglutinin-15(Siglec-15)promotes hepatocellular carcinoma proliferation and migration by regulating the signal transducer and activator of transcription 1/3(STAT1/STAT3)pathway.Methods SMMC-7721 and BEL-7402 cells were divided into empty vector groups:S-NC and B-NC groups(NC siRNA was transfected into SMMC-7721 and BEL-7402 cell,respectively),Siglec-15 knockdown expression group:S-si-Siglec-15-3 and B-si-Siglec-15-3 groups(Siglec-15-3 siRNA was transfected into SMMC-7721 and BEL-7402 cell,respectively).The proliferation ability of the cells were detected by methyl thiazolyl tetrazolium(MTT);the longitudinal and lateral migration ability of the cells were detected by Transwell and scratch assay;the expression of STAT3,phosphorylation of STAT3(p-STAT3),ST AT1,phosphorylation of STAT1(p-STAT1),Siglec-15 was detected by Western blot.Results Cell viability values at 24 h in the S-NC group,S-si-Siglec-15-3 group,B-NC group and B-si-Siglec-15-3 group were 0.93±0.01,0.87±0.03,0.96±0.03 and 0.83±0.02,respectively;the vertical migration rates of cells were 100.00％,36.40％,100.00％and 52.20％,respectively;the healing rate of cell scratches at 24 h were(0.76±0.02)％,(0.53±0.02)％,(0.44±0.02)％and(0.36±0.03)％,respectively;STAT3 protein expression levels were 1.11±0.05,0.66±0.05,1.07±0.14 and 0.49±0.04,respectively;p-STAT3 protein expression levels were 1.07±0.04,0.16±0.06,0.78±0.05 and 0.32±0.03,respectively;STAT1 protein expression levels were 1.32±0.04,0.71±0.04,0.91±0.03 and 0.61±0.04,respectively;p-ST AT1 protein expression levels were 0.99±0.04,0.29±0.05,0.67±0.04 and 0.20±0.06,respectively;Siglec-15 protein expression levels were 1.51±0.04,0.93±0.05,0.73±0.02 and 0.12±0.01,respectively.The differences between the S-si-Siglec-15-3 group and the S-NC group,between the B-si-Siglec-15-3 group and the B-NC group were statistically significant in all comparisons(P＜0.05,P＜0.01,P＜0.001).Conclusion Siglec-15 promotes the proliferation and migration ability of hepatocellular carcinoma cells through the STAT1/STAT3 pathway.关键词
唾液酸结合性免疫球蛋白样凝集素-15/信号传导与转录激活因子1/3通路/肝癌/增殖/迁移Key words
salivary acid-binding immunoglobulin-like agglutinin-15/signal transducer and activator of transcription 1/3 pathway/hepatocellular carcinoma/proliferation/migration分类
医药卫生引用本文复制引用
闫家伟,关璐璐,郭彩茹,王金艳,张亚丽,文学军,金自立,亓民..Siglec-15调节STAT1/STAT3通路促进肝癌细胞增殖和迁移的研究[J].中国临床药理学杂志,2025,41(3):350-354,5.基金项目
河南省医学科技攻关计划联合共建基金资助项目(LHGJ20230818) （LHGJ20230818）
