Abstract
Objective To investigate the effect of venetoclax(Vene)on cell proliferation and apoptosis in acute myeloid leukemia(AML)by regulating the microRNA-410-3p(miR-410-3p)/high mobility group protein B1(HMGB1)axis.Methods THP1 cells were randomly divided into control group(normal culture),experimental group(100 nmol·L-1 Vene treatment),inh-NC group(experimental group+transfection of inh-miR-410-3p negative control),inh-miR-410-3p group(experimental group+transfection of inh-miR-410-3p),inh-NC combined with sh-NC group(inh-NC group+transfection of sh-HMGB1 negative control),inh-miR-410-3p combined with sh-NC group(inh-miR-410-3p group+transfection of sh-HMGB1 negative control),sh-HMGB1 group(inh-miR-410-3p group+transfection of sh-HMGB1).The relative expression levels of miR-410-3p and HMGB1 mRNA were detected by real-time quantitative polymerase chain reaction;immunofluorescence assay was used to detect the positive expression of HMGB1;5-ethynyl-2'-deoxyuridine(EdU)was used to detect cell proliferation;Western blot was used to detect the relative expression levels of B lymphocytoma-2(Bcl-2)and Bcl-2 associated X protein(Bax).Results The relative expression levels of miR-410-3p in the control group,experimental group,inh-NC group,inh-miR-410-3p group,inh-NC combined with sh-NC group,inh-miR-410-3p combined with sh-NC group and sh-HMGB1 group were 1.00±0.15,3.76±0.31,3.64±0.33,1.69±0.20,1.00±0.17,0.42±0.06,0.89±0.07,respectively;the relative expression levels of HMGB1 mRNA were 1.00±0.13,0.62±0.07,0.65±0.06,0.93±0.11,1.00±0.15,1.93±0.21,1.14±0.16,respectively.The relative fluorescence levels of HMGB1 in the control group,experimental group,inh-NC group and inh-miR-410-3p group were 1.00±0.16,0.55±0.07,0.58±0.08,0.82±0.14,respectively.The cell proliferation rates of inh-NC combined with sh-NC group,inh-miR-410-3p combined with sh-NC group and sh-HMGB1 group were(11.46±1.95)％,(37.82±5.31)％,(16.14±3.05)％,respectively;the relative expression levels of Bcl-2 protein were 1.00±0.14,4.17±0.39,1.33±0.18,respectively;the relative expression levels of Bax protein were 1.00±0.16,0.32±0.08,0.91±0.13,respectively.The differences of above indexes were statistically significant between control group and the experimental group,between inh-NC group and the inh-miR-410-3p group,between inh-NC combined with sh-NC group and inh-miR-410-3p combined with sh-NC group,between inh-miR-410-3p combined with sh-NC group and the the sh-HMGB1 group(all P＜0.001).Conclusion Vene can inhibit the proliferation of AML cells and induce apoptosis of AML cells by regulating the miR-410-3p/HMGB1 axis.关键词
维奈克拉/急性髓性白血病/微小RNA/高迁移率族蛋白B1/B淋巴细胞瘤-2/细胞凋亡Key words
venetoclax/acute myeloid leukemia/microRNA/high mobility group protein/B-cell lymphoma-2/cell apoptosis分类
医药卫生
