中国临床药理学杂志2025，Vol.41Issue(3)：361-365,5.DOI:10.13699/j.cnki.1001-6821.2025.03.013
去氢骆驼蓬碱体外抗细粒棘球蚴的非靶向代谢组学研究
Non-targeted metabolomics research of harmine anti-Echinococcus granulosus in vitro
摘要
Abstract
Objective To investigate the mechanism of action of harmine(HM)in anti-Echinococcus granulosus(E.g)based on non-targeted metabolomics.Methods The aseptically cultured fine-grained E.g was divided into blank group(KB group,conventional culture),solvent group(DMSO group,DMSO 2 μL)and administration group(HM group,20 mmoL·L-1 HM solution 2 μL).Survival rate was observed by eosin staining after 48 h of intervention and the ultrastructural changes were observed by scanning electron microscopy,respectively.Meanwhile,non-targeted metabolomics analysis was performed based on ultra performance liquid chromatography-mass spectrometry(UPLC-MS/MS)technology on each group of E.g to screen the differential metabolites and explore the effect of harmine on the metabolic profile of E.g.Results Eosin staining and scanning electron microscopy results showed that harmine had a significant inhibitory effect on E.g in vitro.Metabolomics results showed that there were a total of 15 metabolites with significant differences between the harmine and blank groups(all P＜0.05).Enrichment of the differential metabolites by Kyoto Encyclopedia of Genes and Genomes(KEGG)showed that harmine mainly affected the metabolic pathways of amino acid metabolism,fatty acid metabolism and nucleotide metabolism in E.g.Conclusion Harmine had a significant inhibitory effect on E.g,the mechanism of which may be related to the regulation of amino acid metabolism,fatty acid metabolism and nucleotide metabolism pathways,which can provide a new way of thinking for the development of the subsequent anti-cystic echinococlosis drug targets.关键词
去氢骆驼蓬碱/非靶向代谢组学/细粒棘球蚴/氨基酸代谢/脂肪酸代谢Key words
harmine/non-targeted metabolomics/Echinococcus granulosus/amino acid metabolism/fatty acid metabolism分类
医药卫生引用本文复制引用
徐勤伟,高惠静,卡地尔亚·库尔班,马芹,滕亮..去氢骆驼蓬碱体外抗细粒棘球蚴的非靶向代谢组学研究[J].中国临床药理学杂志,2025,41(3):361-365,5.基金项目
省部共建中亚高发病成因与防治国家重点实验室开放课题基金资助项目(SKL-HIDCA-2022-BC3,SKL-HIDCA-2023-YX1) （SKL-HIDCA-2022-BC3,SKL-HIDCA-2023-YX1）
新疆维吾尔自治区重点研发基金资助项目(2022B03013-4) （2022B03013-4）
新疆维吾尔自治区自然科学基金资助项目(2023D01D16,2023D01A120) （2023D01D16,2023D01A120）
中华国际医学交流会基金会-中华医学会临床药学分会临床药学科研基金资助项目(Z-2021-46-2101-2023) （Z-2021-46-2101-2023）
兵团科技攻关计划基金资助项目(2023AB009-04) （2023AB009-04）
