中国临床药理学杂志2025，Vol.41Issue(3)：366-371,6.DOI:10.13699/j.cnki.1001-6821.2025.03.014
马钱苷减轻糖尿病视网膜病变大鼠视网膜病变的研究
Research of loganin alleviates retinopathy in diabetic retinopathy rats
摘要
Abstract
Objective To investigate the effect and mechanism of loganin on retinopathy in diabetic retinopathy(DR)rats.Methods DR rats were established by injection of streptozotocin and high glucose and high fat.They were randomly grouped into model group(0.9％NaCl),experimental-L group,experimental-H group(50,100 mg·kg-1 loganin),combined group[100 mg·kg-1 loganin+10 mg·kg-1 lysophosphatidic acid(LPA)]and 10 normal rats were taken as control group(0.9％NaCl).After the intervention,retinal histomorphology,the levels of inflammatory factors and oxidative stress,apoptosis of ganglion cells,and the expression of Ras homologous gene family member A(RhoA)and Rho associated coiled coil-forming kinase(ROCK)proteins in retinal tissue were measured in turn.Results The number of retinal ganglion cells in experimental-L,-H groups,combined group,model group and control group were 19.75±1.90,24.32±2.15,17.80±1.94,15.44±1.65 and 28.30±2.02;the levels of interleukin-6 were(85.67±8.61),(68.88±6.91),(98.52±9.88),(108.24±10.83)and(62.08±6.22)ng·L-1;the levels of tumor necrosis factor-α were(150.24±15.11),(110.38±11.06),(186.94±18.77),(200.05±20.08)and(105.34±10.55)ng·L-1;the levels of malondialdehyde were(7.52±0.78),(4.76±0.48),(9.52±0.96),(10.85±1.09)and(4.58±0.46)μmol·mg-1;the superoxide dismutase activities were(62.05±6.21),(78.22±7.83),(52.11±5.23),(50.21±5.04)and(79.61±7.98)U·mg-1;the apoptosis rates of ganglion cells were(12.71±1.06)％,(6.98±0.93)％,(14.69±1.30)％,(19.50±1.42)％and(2.15±0.38)％;the relative expression levels of RhoA protein were 0.81±0.09,0.42±0.05,1.08±0.11,1.16±0.12 and 0.38±0.04;the relative expression levels of ROCK protein were 1.28±0.13,0.77±0.08,1.72±0.18,1.85±0.19 and 0.63±0.07,respectively.Compared with the model group,the above indexes of the control group,experimental-H group and combination group had statistical significance(all P＜0.05).Conclusion Loganin may improve retinopathy in DR rats by inhibiting the activation of RhoA/ROCK pathway,inhibiting inflammation and oxidative stress response.关键词
马钱苷/糖尿病视网膜病变/Ras同源基因家族成员A/Rho相关的卷曲螺旋激酶/氧化应激/炎症反应Key words
loganin/diabetic retinopathy/Ras homologous gene family member A/Rho associated coiled coil-forming kinase/oxidative stress/inflammatory response分类
医药卫生引用本文复制引用
徐林丽,闫萌萌,刘岩岩,张艳..马钱苷减轻糖尿病视网膜病变大鼠视网膜病变的研究[J].中国临床药理学杂志,2025,41(3):366-371,6.基金项目
河北省沧州市重点研发指导基金资助项目(192106021) （192106021）
