中国临床药理学杂志2025，Vol.41Issue(3)：372-375,4.DOI:10.13699/j.cnki.1001-6821.2025.03.015
微小RNA-142a-3p通过靶向下调Atg16l1表达介导阿霉素肾病的研究
Research of microRNA-142a-3p mediates adriamycin-induced nephropathy by targeting down-regulation of Atg16l1 expression
摘要
Abstract
Objective To explore effect and mechanism of microRNA 142a-3p(miR-142a-3p)on adriamycin-induced nephropathy(AN).Methods BALB/c mice were randomly divided into normal group and model group.The model group was injected with 10.5 mg·kg-1 doxorubicin at a time through the tail vein,and the normal group was injected with equal volume 0.9％NaCl by the same method.After 28 days of feeding,both sides of the kidney tissues were taken for experiment.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of miR-142a-3p in the renal tissues.The expression levels of autophagy related protein 16-like protein 1(Atg16l1)and inflammatory NOD-like receptor heat protein domain associated protein 3(NLRP3),interleukin-1β(IL-1 β)and interleukin-18(IL-18)in the renal tissues were detected by Western blotting.Human embryonic kidney 293T cells were divided into Atg16l1-WT+NC transfection group(transfected pmirGLO-Atg16l1-WT and NC mimics)and Atg16l1-WT+miRNA-142a-3p group(transfected pmirGLO-Atg16l1-WT and miR-142a-3p mimics).Dual luciferase assay was used to detect the targeted regulation of miR-142a-3p on Atg16l1.Results The expression levels of miR-142a-3p in kidney tissues of normal group and model group were 1.20±0.27 and 2.02±0.14;the expression levels of Atg16l1 were 0.81±0.13 and 0.57±0.12,NLRP3 were 0.49±0.11 and 0.82±0.14;IL-1 β were 0.55±0.11 and 0.84±0.08;IL-18 levels were 0.41±0.15 and 0.74±0.09,respectively,and the above indexes in the model group were significantly different from those in the normal group(all P＜0.05).The relative luciferase activity in Atg16l1-WT+NC transfection group and Atg16l1-WT+miRNA-142a-3p transfection group were 1.26±0.07 and 0.92±0.10,respectively,with statistical significance(all P＜0.05).Conclusion MiR-142a-3p down-regulates the expression of target protein Atg16l1 in renal tissue of adriamycin nephropathy mice,which activates NLRP3 pathway of inflammatory bodies and promotes the progressive development of adriamycin-induced nephropathy.关键词
阿霉素肾病/微小RNA-142a-3p/自噬相关蛋白16样蛋白1/炎症小体Key words
adriamycin-induced nephropathy/microRNA-142a-3p/autophagy associated protein 16-like protein-1/inflammatory body分类
医药卫生引用本文复制引用
袁瑞阳,丁海麦,赵星,苏力德,张学明..微小RNA-142a-3p通过靶向下调Atg16l1表达介导阿霉素肾病的研究[J].中国临床药理学杂志,2025,41(3):372-375,4.基金项目
内蒙古自治区自然科学基金资助项目(2020MS08015、2020MS08007) （2020MS08015、2020MS08007）
内蒙古自治区留学回区人员创新启动支持计划基金资助项目(2021) （2021）
内蒙古自治区科技创新引导奖励基金资助项目(Z19SQ2-CZT01) （Z19SQ2-CZT01）
包头医学院花蕾计划基金资助项目(HLJH202315) （HLJH202315）
