中国临床药理学杂志2025，Vol.41Issue(3)：376-380,5.DOI:10.13699/j.cnki.1001-6821.2025.03.016
敦煌泻心汤对溃疡性结肠炎小鼠的治疗作用
Therapeutic effects of Dunhuang Xiexin decoction on mice with ulcerative colitis
摘要
Abstract
Objective To explore the therapeutic effects and mechanisms of Dunhuang Xiexin decoction on mice with ulcerative colitis(UC).Methods Mice were randomly divided into blank group(0.9％NaCl),model group(0.9％NaCl)and experimental-L,-M,-H groups(2.93,5.85,11.70 g·kg-1 Dunhuang Xiexin decoction water extracts)with 10 cases per group for 7 days.Meanwhile,apart from the blank group,the other four groups of mice were allowed adlibitum access to drinking water containing 3％sodium dextran sulfate for 7 consecutive days to establish the UC model.The levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1 β)and IL-6 in mouse serum were determined by enzyme-linked immunosorbent assay.The expression levels of occludin,zonula occludens-1(ZO-1)and phospho nuclear factor kappa B p65(p-NF-κB p65)in colonic tissues was detected by Western blot.Results The serum TNF-α levels in the experimental-M,-H groups,model group and blank group were(397.57±33.05),(389.41±22.57),(472.19±18.70)and(355.92±31.86)pg·mL-1;the IL-1 β levels were(73.06±18.38),(62.60±9.80),(98.94±27.50)and(55.26±18.14)ng·mL-1;the IL-6 levels were(153.23±32.96),(148.12±23.53),(239.35±50.99)and(131.36±27.58)pg·mL-1;the relative expressions levels of occludin protein in colon tissues were 0.80±0.13,1.03±0.24,0.57±0.15 and 1.10±0.03;the relative expressions levels of ZO-1 protein were 0.90±0.21,1.09±0.10,0.73±0.16 and 1.29±0.03;the relative expressions levels of p-NF-κB p65 protein were 0.75±0.12,0.53±0.08,1.15±0.19 and 0.43±0.21,respectively.There were significant differences between the experimental-H group and the model group(P＜0.05).Conclusion Dunhuang Xiexin decoction may improve ulcerative colitis in mice by repairing the intestinal barrier and inhibiting the NF-κB signaling pathway to reduce the release of inflammatory factors.关键词
敦煌泻心汤/敦煌医学/溃疡性结肠炎/葡聚糖硫酸钠/肠道屏障/核因子-κBKey words
Dunhuang Xiexin decoction/Dunhuang medicine/ulcerative colitis/dextran sulfate sodium/intestinal barrier/nuclear factor kappa-B分类
医药卫生引用本文复制引用
杜籼芹,高榕妮,李爽,李亮亮,蔺兴遥..敦煌泻心汤对溃疡性结肠炎小鼠的治疗作用[J].中国临床药理学杂志,2025,41(3):376-380,5.基金项目
甘肃省人才发展专项基金资助项目(2021RCXM106) （2021RCXM106）
