中国临床药理学杂志2025，Vol.41Issue(3)：381-386,6.DOI:10.13699/j.cnki.1001-6821.2025.03.017
基于EGFR/PI3K/AKT信号通路探讨延胡索乙素对子宫内膜异位症痛经SD大鼠的作用
Effects of Tetrahydropalmatine on endometriosis dysmenorrhoea in SD rats based on EGFR/PI3K/AKT signaling pathway
摘要
Abstract
Objective To observe the effect of Tetrahydropalmatine on the epidermal growth factor receptor/phosphatidylinositol 3-kinase/protein kinase B(EGFR/PI3K/AKT)signaling pathway in endometriosis dysmenorrhoea SD rats.Methods The endometriosis dysmenorrhoea model was replicated by auto-transplantation in 60 female SD rats of SPF grade.After successful modelling,the rats were randomly divided into model group,control group and experimental-L,-M,-H groups according to body mass,with 12 rats in each group;and another 12 normal rats were taken as the blank group.The blank and model groups were given 10 mL·kg-1 0.9％NaCl by gavage,and the control group was given 0.25 mg·kg-1 progesterone suspension by gavage.The experimental-L,experimental-M,experimental-H groups were given 10,20 and 40 mg·kg-1 Tetrahydropalmatine suspension by gavage,respectively.Six groups of rats were administered once daily for 4 weeks.After the last administration,2 U of oxytocin was given to induce contractions,and the rats were observed for torsional responses.The serum levels of tumor necrosis factor-α(TNF-α),epidermal growth factor(EGF),and EGFR were measured by enzyme-linked immunosorbent assay in each group of rats,and the expression levels of EGFR,PI3K and AKT proteins in ectopic endometrial tissues were detected by Western blotting.Results The number of twists in the experimental-M,-H groups and control group,model group,blank group were 9.25±1.42,8.33±1.88,11.17±2.41,28.67±2.15 and 0;the inhibition rates were 67.74％,70.95％,61.05％,0 and 0;serum TNF-αlevels were(281.96±13.06),(278.75±10.39),(282.12±14.47),(303.59±9.09)and(274.26±15.33)ng·L-1;serum EGF levels were(313.82±13.23),(308.26±10.90),(311.39±19.52),(336.04±17.60)and(301.67±27.17)pg·mL-1;serum EGFR levels were(315.71±7.56),(311.43±11.04),(313.75±7.26),(333.56±14.22)and(307.86±17.94)ng·L-1;the relative expression levels of EGFR protein were 1.60±0.39,1.26±0.30,1.40±0.40,2.08±0.41 and 1.00±0.22;the relative expression levels of PI3K protein were 1.60±0.42,1.21±0.18,1.27±0.24,2.01±0.41 and 1.00±0.19;the relative expression levels of AKT protein were 1.27±0.18,1.11±0.10,1.27±0.16,1.64±0.28 and 1.00±0.07,respectively.Statistically significant differences were found between the above indicators in the experimental-M,-H groups compared to the model group(P＜0.01,P＜0.05).Conclusion Tetrahydropalmatine may play a therapeutic role in endometriosis dysmenorrhoea by interfering with the EGFR/PI3K/AKT signaling pathway.关键词
延胡索乙素/子宫内膜异位症/痛经/表皮生长因子受体/磷酸肌醇3-激酶/蛋白激酶B信号通路Key words
Tetrahydropalmatine/endometriosis/dysmenorrhoea/epidermal growth factor receptor/phosphatidylinositol 3-kinase/protein kinase B signaling pathway分类
医药卫生引用本文复制引用
张作良,王婉润,林祥羽,王家兴,胡安鑫,张志瑞,杨亚玲,武权生..基于EGFR/PI3K/AKT信号通路探讨延胡索乙素对子宫内膜异位症痛经SD大鼠的作用[J].中国临床药理学杂志,2025,41(3):381-386,6.基金项目
国家自然科学基金资助项目(82260949) （82260949）
甘肃省自然科学基金资助项目(23JRRA1199) （23JRRA1199）
甘肃省中医药科研基金资助项目(GZKP-2022-22) （GZKP-2022-22）
兰州市科技计划基金资助项目(2021-1-97) （2021-1-97）
甘肃中医药大学附属医院院内课题基金资助项目(gzfy-2021-03) （gzfy-2021-03）
