中国临床药理学杂志2025，Vol.41Issue(3)：397-402,6.DOI:10.13699/j.cnki.1001-6821.2025.03.020
炙红芪对脾气虚大鼠盲肠中短链脂肪酸代谢水平的影响
Effects of honey-processed Hedysari Radix on short-chain fatty acid metabolism in cecum of rats with spleen qi deficiency
摘要
Abstract
Objective To explore the effect of honey-processed Hedysari Radix on the metabolism of short-chain fatty acids(SCFAs)in the cecum of rats with spleen qi deficiency.Methods A total of 48 SPF male SD rats were randomly divided into blank group,model group,control group and experimental group,with 8 rats in each group.The blank group was fed normally,drank water and ate freely for 15 days,and the other groups used the three-factor composite modeling method of bitter cold diarrhea,overwork and hunger and satiety to construct a spleen qi deficiency model rats for 15 days.After the successful replication of the model,the rats in the control group were given 62.5 mg·kg-1 bifidobacterium Lactobacillus triple viable tablets suspension,the experimental group was given 12.6 g·kg-1 honey-processed Hedysari Radix decoction,and the blank group was given the same dose of distilled water,and the model group continued to model for 15 days.Gas chromatography-mass spectrometry(GC-MS)was used to determine the contents of SCFAs in the cecal contents of rats in each group.Results The contents of acetic acid,isobutyric acid,butyric acid,isovaleric acid and caproic acid in the cecal contents of rats in the blank group were(3 706.24±401.84),(99.34±39.11),(2 567.95±529.44),(86.50±54.11)and(393.14±103.33)μg·g-1,respectively.Compared with the blank group,the contents of acetic acid,isobutyric acid,butyric acid,isovaleric acid and caproic acid in the cecum contents of spleen-qi deficiency rats in the model group were significantly reduced,which were(3 049.48±590.62),(67.27±12.99),(2 058.00±417.46),(43.10±11.12)and(27.98±14.40)μg·g-1,respectively(P＜0.05,P＜0.01).Compared with the model group,the contents of isovaleric acid and caproic acid in the cecum contents of rats in the experimental group were significantly increased,which were(90.59±26.03)and(60.05±19.89)μg·g-1,respectively(all P＜0.01).Conclusion Honey-processed Hedysari Radix can play a role in strengthening the spleen and replenishing qi by improving the metabolism level of cecal contents,regulating the metabolism levels of SCFAs in cecal contents,protecting the intestinal mucosal barrier,and reducing intestinal inflammation.关键词
炙红芪/脾气虚证/气相色谱质谱联用法/短链脂肪酸/肠黏膜屏障Key words
honey-processed Hedysari Radix/spleen qi deficiency/gas chromatography-mass spectrometry/short-chain fatty acid/intestinal mucosal barrier分类
医药卫生引用本文复制引用
李越峰,严兴科,宋沁洁,王毛毛,王哲,马定财,张育贵,刘婷,辛二旦,孙宇靖..炙红芪对脾气虚大鼠盲肠中短链脂肪酸代谢水平的影响[J].中国临床药理学杂志,2025,41(3):397-402,6.基金项目
国家自然科学基金资助项目(82160750) （82160750）
甘肃省教育厅产业支撑计划基金资助项目(2021CYZC-21) （2021CYZC-21）
甘肃省科技计划(创新基地与人才计划)基础研究创新群体基金资助项目(21JR7RA569) （创新基地与人才计划）
甘肃省重点人才项目 （）
甘肃省联合科研基金资助项目 （）
高等学校产业支撑计划基金资助项目(2024CYZC-41) （2024CYZC-41）
