中国临床药理学杂志2025，Vol.41Issue(4)：477-481,5.DOI:10.13699/j.cnki.1001-6821.2025.04.006
去氧地胆草素调控非小细胞肺癌进展的机制研究
Study on the mechanism of deoxyelephantopin regulating the progression of non-small cell lung carcinoma
摘要
Abstract
Objective To investigate the inhibitory effect of deoxyelephantopin on non-small cell lung cancer(NSCLC)and its potential mechanism.Methods Human lung adenocarcinoma H1975 cells were randomly divided into control group(routine culture),experimental-L group(1.0 μmol·L-1 deoxyelephantopin),experimental-M group(2.0 μmol·L-1 deoxyelephantopin),experimental-H group(5.20 μmol·L-1 deoxyelephantopin),Vector group(transfected Vector plasmid+5.20 μmol·L-1 deoxyelephantopin),epidermal growth factor receptor(EGFR)group(transfected with EGFR plasmid+5.20 μmol·L-1 deoxyelephantopin).5-ethynyl-2'-deoxyuridine(EdU)assay was used to detected cell proliferation;cell apoptosis were detected by TdT mediated dUDP nick end labeling(Tunel)and flow cytometry;Western blot assay was used to detect the proteins expression;and Transwell assay was used to detect cell migration and invasion.Results The proliferation rates of cells in the control group and experimental-L,-M,-H groups were(40.64±2.70)％,(29.78±3.28)％,(23.59±1.98)％and(15.72±1.01)％,respectively;the rates of TUNEL-positive cells were(4.63±0.57)％,(10.18±0.88)％,(15.96±0.79)％and(24.73±3.70)％,respectively;the relative expression levels of phosphorylated epidermal growth factor receptor(p-EGFR)protein were 0.87±0.10,0.60±0.05,0.49±0.04 and 0.33±0.04,respectively.Comparisons between the experimental-L,-M,-H groups and the control group,as well as comparisons among the experimental-L,-M,-H groups,revealed statistically significant differences(all P＜0.05).The relative expression levels of p-EGFR/EGFR protein in the control group,experimental-H group,Vector group,and EGFR group were 0.76±0.10,0.49±0.07,0.47±0.05 and 0.71±0.10,respectively;apoptosis rates were(4.11±0.39)％,(26.33±2.29)％,(25.40±1.58)％and(12.68±1.73)％,respectively;the number of migrating cells was 147.78±12.69,87.11±9.65,93.89±9.67 and 124.00±14.10,respectively.Statistical analysis showed significant differences in the above indices between the experimental-H group and the control group,as well as between the EGFR group and the Vector group(all P＜0.05).Conclusion Deoxy elephantopin can degrade EGFR,inhibit related signaling pathways,inhibit proliferation and metastasis of lung cancer cells,and induce apoptosis.关键词
去氧地胆草素/非小细胞肺癌/表皮生长因子受体/增殖/凋亡/转移Key words
deoxyelephantopin/non-small cell lung cancer/epidermal growth factor receptor/proliferation/apoptosis/transfer分类
中医学引用本文复制引用
席彩霞,陶文学,刘雪梅,张文静,张海慧,张文博..去氧地胆草素调控非小细胞肺癌进展的机制研究[J].中国临床药理学杂志,2025,41(4):477-481,5.基金项目
武威市市级科技计划自筹经费基金资助项目(WW23B02SF038) （WW23B02SF038）
