中国临床药理学杂志2025，Vol.41Issue(4)：482-486,5.DOI:10.13699/j.cnki.1001-6821.2025.04.007
汉黄芩苷上调miR-573表达抑制胰腺癌SW1990细胞增殖、迁移及侵袭的研究
Research of wogonoside inhibiting the proliferation,migration and invasion of pancreatic cancer SW1990 cells by up-regulating the expression of miR-573
摘要
Abstract
Objective To investigate the effects and mechanism of wogonoside on the malignant biological behavior of pancreatic cancer SW1990 cells.Methods Human pancreatic cancer cells SW1990 were cultured in vitro and randomly divided into control group(normal culture),miR-NC group(transfected with miR-NC),miR-573 group(transfected with miR-573 mimics),anti-miR-NC group(transfected with miR-NC+20 μmol·L-1 wogonoside),anti-miR-573 group(transfected with anti-miR-573+20 μmol·L-1wogonoside)and experimental-L,-M,-H groups(given 5,10 and 20 μmol·L-1wogonoside,respectively).The ability of cell proliferation,migration and invasion were detected by methyl thiazolyl tetrazolium assay,plate cloning assay,scratch assay and Transwell assay,respectively;the expression level of microRNA(miR-573)was detected by real-time fluorescence quantitative polymerase chain reaction;Western blot were used to detect the expression levels of E-cadherin and N-cadherin protein.Results The inhibition rates of experimental-H group,control group,miR-NC group,miR-573 group,anti-miR-NC group and anti-miR-573 group were(65.37±5.17)％,0,(6.13±0.58)％,(51.94±4.99)％,(67.74±4.38)％and(28.23±2.47)％,respectively;the scratch healing rates were(27.33±2.52)％,(73.47±6.56)％,(74.27±6.86)％,(36.42±3.14)％,(25.91±2.53)％and(61.49±5.11)％,respectively;the number of invading cells were 58.05±5.46,124.21±10.25,126.77±11.89,64.74±5.63,56.65±4.79 and 103.94±10.68,respectively;the relative expression levels of miR-573 were 3.11±0.26,1.00±0.00,1.00±0.00,3.96±0.37,1.00±0.00 and 0.35±0.03,respectively;the relative expression levels of E-cadherin protein were 0.56±0.04,0.16±0.02,0.15±0.02,0.50±0.04,0.58±0.04 and 0.28±0.03,respectively;the relative expression levels of N-cadherin protein were 0.24±0.02,0.66±0.04,0.68±0.05,0.31±0.03,0.23±0.02 and 0.55±0.05,respectively.The differences of above indexes were statistically significant between the experimental-H group and the control group,between the miR-573 group and the miR-NC group,and between the anti-miR-573 group and the anti-miR-NC group(all P＜0.05).Conclusion Wogonoside can decrease the proliferation,migration and invasion of pancreatic cancer cells by up-regulating the expression of miR-573,decreasing the expression of N-cadherin and increasing the expression of E-cadherin.关键词
汉黄芩苷/胰腺癌/微小RNA-573/细胞增殖/迁移/侵袭Key words
wogonoside/pancreatic cancer/microRNA-573/cell proliferation/migration/invasion分类
医药卫生引用本文复制引用
王晨曦,陈中建,延学军,刘洪锋,马铎,刘瑶,李永坤..汉黄芩苷上调miR-573表达抑制胰腺癌SW1990细胞增殖、迁移及侵袭的研究[J].中国临床药理学杂志,2025,41(4):482-486,5.基金项目
南阳市科技攻关计划基金资助项目(KJGG150) （KJGG150）
