中国临床药理学杂志2025，Vol.41Issue(4)：497-501,5.DOI:10.13699/j.cnki.1001-6821.2025.04.010
当归红芪超滤物对放射性心脏病大鼠的影响
Effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration on radiation heart disease in rats
摘要
Abstract
Objective To observe the intervention effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration(RAS-RH)on the NOD-like receptor thermal protein domain associated protein 3/cysteinyl aspartate specific proteinase-1/gasdermin D(NLRP3/Caspase-1/GSDMD)signaling pathway and radiation-induced heart disease(RIHD).Methods A model was constructed using a single whole-body X-ray irradiation at a dose of 8 Gy.SPF-grade male Wistar rats were randomly divided into the normal group,the model group(X-ray 8 Gy),the experimental-L group(X-ray 8 Gy+25 mg·kg-1·d-1 RAS-RH by gavage)and the experimental-H group(X-ray 8 Gy+50 mg·kg-1·d-1 RAS-RH by gavage),with ten rats in each group.2 h after the last administration,cardiac function was evaluated using echocardiography;serum levels of inflammatory factors,including interleukin-18(IL-18),IL-1 β and tumor necrosis factor-alpha(TNF-α)were measured using enzyme-linked immunosorbent;relative protein expression levels of NLRP3,Caspase-1,GSDMD,IL-18 and IL-1 β in myocardial tissue were detected using Western blotting.Results The left ventricular ejection fraction(LVEF)in experimental-L group,experimental-H group,normal group and model group were(66.61±1.04)％,(71.89±0.69)％,(81.45±1.34)％and(47.62±1.67)％;the serum levels of IL-18 were(171.21±3.92),(145.60±3.37),(125.51±2.94)and(201.80±4.05)ng·mL-1;the IL-1β levels were(43.03±0.68),(41.12±1.01),(36.12±0.98)and(51.19±0.85)ng·mL-1;the TNF-α levels were(484.96±3.35),(441.24±3.94),(381.32±4.16)and(566.51±4.98)ng·mL-1;the relative expression levels of NLRP3 protein in myocardial tissue were 0.54±0.02,0.34±0.02,0.20±0.02 and 0.75±0.03;the relative expression levels of Caspase-1 protein were 0.52±0.02,0.44±0.02,0.36±0.02 and 0.65±0.03;the relative expression levels of GSDMD-NT protein were 0.53±0.02,0.42±0.01,0.33±0.02 and 0.73±0.03;the relative expression levels of IL-18 protein were 0.50±0.02,0.42±0.01,0.13±0.01 and 0.63±0.02;the relative expression levels of IL-1βprotein were 0.59±0.02,0.42±0.02,0.27±0.02 and 0.79±0.03,respectively.The indicators in the model group were significantly different compared to the normal group,and the indicators in the experimental-L and experimental-H groups were significantly different from the model group,with statistical significance(all P＜0.05).Conclusion RAS-RH may improve RIHD by regulating the NLRP3/Caspase-1/GSDMD signaling pathway.关键词
当归红芪超滤物/放射性心脏病/NOD样受体热蛋白结构域相关蛋白3/半胱氨酸天冬氨酸蛋白酶-1/消皮素D信号通路Key words
Radix Angelica Sinensis and Radix Hedysari ultrafiltration/radiation-induced heart disease/NOD-like receptor protein 3/Caspase-1/Gasdermin D signaling pathway分类
医药卫生引用本文复制引用
高翔,卢国赐,任春贞,吕欣芳,陈其林,吴雪,支晓东,王春玲,李应东..当归红芪超滤物对放射性心脏病大鼠的影响[J].中国临床药理学杂志,2025,41(4):497-501,5.基金项目
国家自然科学基金资助项目(82260869) （82260869）
甘肃省自然科学基金资助项目(22JR11RA128) （22JR11RA128）
甘肃教育揭榜挂帅基金资助项目(2021jyjbgs-03) （2021jyjbgs-03）
