中国临床药理学杂志2025，Vol.41Issue(4)：502-506,5.DOI:10.13699/j.cnki.1001-6821.2025.04.011
基于NRF2/NLRP3/焦亡轴探讨丹参酮ⅡA抑制心肌梗死的作用机制
Mechanism of Tanshinone ⅡA on the inhibition of myocardial infarction based on NRF2/NLRP3/pyroptosis axis
摘要
Abstract
Objective To explore the underlying mechanism of Tanshinone Ⅱ A(Tan ⅡA)exerted in myocardial infarction(MI)through nuclear factor E2-related factor 2(NRF2)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)/pyroptosis axis.Methods SD rats were randomly divided into sham operation group[only exposed the heart without left anterior descending(LAD)ligation],model group(LAD ligation)and experimental group(LAD ligation and 10 mg·kg-1 Tan ⅡA).2,3,5-Triphenyte-trazoliumchloride(TTC)staining was used to detect the area of infarction;Western blot was utilized to investigate the expression level of NRF2,NLRP3 and gasdermin D(GSDMD)in myocardial tissue.The H9c2 cells were divided into blank group(normal culture),model group[oxygen-glucose deprivation(OGD)culture],control group(normal culture and 40 μmol·L-1 Tan ⅡA)and combined group(OGD culture and 40 μmol·L-1 Tan ⅡA).Western blot was utilized to investigate the expression level of NRF2,GSDMD in cardiomyocyte.Creatine kinase MB(CK-MB)in cell culture supernatant were detected by automatic biochemical analyzer.Results The proportion of myocardial infarction area in the sham operation group,model group and experimental group were 0,(58.64±13.41)％and(41.69±8.73)％,respectively;the relative expression of NLRP3 protein were 1.01±0.10,2.12±0.26 and 1.48±0.11,respectively;the relative expression of GSDMD protein were 1.03±0.17,2.22±0.20 and 1.40±0.17,respectively;the relative expression of NRF2 protein were 1.12±0.29,0.51±0.02 and 0.96±0.10,respectively;the indicators of the model group compared with the sham operation group,and the indicators of the experimental group were compared with the model group,the differences were statistically significant(all P＜0.05).The CK-MB in blank group,model group,control group and combined group were(54.30±19.24),(208.60±38.19),(46.76±13.63)and(126.10±42.87)U·L-1,respectively;the relative expression of NRF2 protein were 1.37±0.14,0.44±0.08,1.34±0.17 and 0.79±0.11,respectively;the relative expression of GSDMD protein were 0.95±0.26,1.97±0.17,1.04±0.20 and 1.16±0.16,respectively.The indicators of the model group compared with the blank group,and the indicators of the combined group were compared with the model group,the differences were statistically significant(all P＜0.05).Conclusion Tan Ⅱ A can alleviate myocardial infarction-induced myocardial injury through regulation NRF2/NLRP3/pyroptosis axis.关键词
丹参酮ⅡA/核因子E2相关因子2/NOD样受体家族蛋白3/心肌梗死/焦亡Key words
Tanshinone Ⅱ A/nuclear factor E2-related factor 2/NOD-like receptor thermal protein domain associated protein 3/myocardial infarction/pyroptosis分类
医药卫生引用本文复制引用
易娜,田源,袁李礼..基于NRF2/NLRP3/焦亡轴探讨丹参酮ⅡA抑制心肌梗死的作用机制[J].中国临床药理学杂志,2025,41(4):502-506,5.基金项目
湖南省自然科学基金资助项目(2022JJ80101,2022JJ80062) （2022JJ80101,2022JJ80062）
湖南省卫健委科研基金资助项目(202203012960,202103010510) （202203012960,202103010510）
湖南省教育厅科研基金资助项目(21B0088) （21B0088）
湖南省中医药科技基金资助项目(D2022024) （D2022024）
