中国临床药理学杂志2025，Vol.41Issue(4)：507-511,5.DOI:10.13699/j.cnki.1001-6821.2025.04.012
荭草花提取物治疗运动性心律失常大鼠的研究
Research of Polygonum orientale flower extract on the rats with exercise arrhythmia
摘要
Abstract
Objective To investigate the effects and mechanism of Polygonum orientale flower(POR)extract on exercise arrhythmia(EA)in rats after exhaustive exercise.Methods The EA rat model was induced by 8 weeks of exhaustive weight-bearing swimming exercise and was randomly divided into model group and experimental-L,-M,-H groups,with 12 rats per group.Twelve normal rats were selected as the normal group.On the modeling day,normal and model groups were given pure water by intragastric administration.Experimental-L,-M,-H groups were given 50,100 and 200 mg·kg-1 POR extract solution by intragastric administration,respectively.Five groups were administrated with once a day for 8 weeks.The latent time and duration of arrhythmia in rats were detected by electrocardiograph.The contents of adenosine triphosphate(ATP),the activities of Na+/K+ATP and the contents of superoxide dismutase(SOD)in myocardial tissue were measured by corresponding kit.The transcriptional levels of calmodulin(CaM)and calmodulin dependent protein kinase Ⅱ(CaMK Ⅱ)in rat myocardium were measured by real-time fluorescence quantitative polymerase chain reaction.Results The latent time of arrhythmia in experimental-M,-H groups and model group were(9.77±1.48),(10.61±1.61)and(7.44±2.12)min;the duration were(33.25±6.39),(20.01±3.89)and(71.44±13.68)min.The ATP contents in experimental-M,-H groups,model group and normal group were(26.45±2.96),(29.44±1.89),(10.38±1.22)and(34.50±4.24)ng·g prot-1;the Na+/K+ATP activities were(17.27±0.92),(19.57±1.33),(5.14±0.80)and(20.00±2.11)U·gprot-1;SOD contents were(89.22±9.07),(86.29±7.22),(73.62±7.36)and(89.43±8.85)U·mg prot-1;the relative expression levels of CaM mRNA in myocardial tissue were 1.28±0.08,1.06±0.08,2.34±0.30 and 1.00±0.10;the relative expression levels of CaMK Ⅱ mRNA in myocardial tissue were 1.46±0.09,1.15±0.09,2.80±0.19 and 1.00±0.11,respectively.Compared with model group,the differences of above indexes in experimental-M,-H groups were statistically significant(all P＜0.05).Conclusion POR extract can effectively treat the rats with EA,and the mechanism is related to the increase of ATP contents,Na+/K+ATP and antioxidant activities,and inhibition of Ca2+/CaM/CaMK Ⅱ signaling pathway.关键词
荭草花提取物/运动性心律失常/Na+/K+腺苷三磷酸酶活性/Ca2+/钙调蛋白/钙调蛋白依赖性蛋白激酶Ⅱ信号通路Key words
Polygonum orientale flower extract/exercise arrhythmia/Na+/K+adenosine triphosphate activity/Ca2+/calmodulin/calmodulin dependent protein kinase Ⅱ signaling pathway分类
医药卫生引用本文复制引用
凌占一,萨如拉,李翠花..荭草花提取物治疗运动性心律失常大鼠的研究[J].中国临床药理学杂志,2025,41(4):507-511,5.基金项目
河北省医学科学研究重点课题计划基金资助项目(20181289) （20181289）
呼伦贝尔市人民医院科研基金资助项目(NMKY2020) （NMKY2020）
