中国临床药理学杂志2025，Vol.41Issue(4)：517-521,5.DOI:10.13699/j.cnki.1001-6821.2025.04.014
丹蛭降糖胶囊改善db/db小鼠肾组织损伤的作用机制
Mechanism of Danzhi Jiangtang capsule in improving kidney tissue injury in db/db mice
摘要
Abstract
Objective To investigate the protective effect of Danzhi Jiangtang capsule on kidney of db/db mice with diabetic kidney disease(DKD)and its effects on the autophagy-lysosomal pathway.Methods The db/db mice were randomly divided into model group(0.2 mL·d-10.9％NaCl),positive control group(45 mg·kg-1·d-1 irbesartan)and experimental-H,-M,-L groups(1 800,900 and 450 mg·kg-1·d-1 Danzhi Jiangtang capsule),and another db/m mice were taken as blank group(0.2 mL·d-1 0.9％NaCl),which were continuously infused for 8 weeks.The expression levels of sequestosome1(P62),microtubule-associated protein light chain 3(LC3),transcription factor EB(TFEB),lysosomal associated membrane protein 1(LAMP-1)and cathepsin D(CTSD)were detected in renal tissues by Western blot.Results The relative expression levels of P62 protein in blank group,model group,positive control group and experimental-H,-M,-L groups were 1.00±0.04,7.66±0.27,3.52±0.11,2.21±0.09,2.86±0.10 and 4.05±0.16;the LC3 Ⅱ/Ⅰ levels were 1.00±0.02,0.17±0.01,0.71±0.08,0.86±0.05,0.80±0.04 and 0.73±0.06;the TFEB protein levels were 1.00±0.01,0.17±0.01,0.30±0.01,0.63±0.01,0.45±0.01 and 0.42±0.01;the LAMP-1 protein levels were 1.00±0.01,0.28±0.02,0.42±0.01,0.77±0.00,0.55±0.03 and 0.40±0.02;the CTSD protein levels were 1.00±0.04,0.27±0.03,0.47±0.04,0.73±0.02,0.63±0.01 and 0.52±0.02.The differences between the above indexes in the experimental-H,-M,-L groups and the model group were statistically significant(all P＜0.01).Conclusion Danzhi Jiangtang capsule can improved renal damage in db/db mice,which may be related to the repair of autophagy-lysosome pathway,accelerate clearance of abnormal proteins and degradation of organelles.关键词
丹蛭降糖胶囊/糖尿病肾病/自噬-溶酶体途径/肾损伤Key words
Danzhi Jiangtang capsule/diabetic kidney disease/autophagy-lysosome pathway/kidney injury分类
医药卫生引用本文复制引用
阮诺冰,方朝晖,李金菊,许奇,李瑜璠,胡可心..丹蛭降糖胶囊改善db/db小鼠肾组织损伤的作用机制[J].中国临床药理学杂志,2025,41(4):517-521,5.基金项目
国家自然科学基金青年基金资助项目(82174153,81573944,81774286) （82174153,81573944,81774286）
中央财政转移支付地方基金资助项目(2023-24) （2023-24）
新安医学与中医药现代化"揭榜挂帅"基金资助项目(2023CXMMTCM003) （2023CXMMTCM003）
