中国临床药理学杂志2025，Vol.41Issue(4)：522-526,5.DOI:10.13699/j.cnki.1001-6821.2025.04.015
红芪多糖对脾虚型DGP大鼠肠道黏膜屏障的保护作用
Protective effect of hedysarum polybotrys polysacchcaide on the intestinal mucosal barrier of rats with spleen deficiency DGP
摘要
Abstract
Objective To investigate the protective effect of hedysarum polybotrys polysacchcaide(HPS)on intestinal mucosal barrier in rats with splenic deficiency type diabetic gastroparesis.Methods The rat model of spleen deficiency DGP was prepared by multifactorial combined with low-dose intraperitoneal injection of Stretocin.The rats were randomly divided into blank group(pure water gavage),model group(pure water gavage),positive control group(0.09 g·kg-1 metformin hydrochloride sustained-release tablet)and experimental-H,-M,-L groups(0.20,0.10,0.05 g·kg-1 HPS),each group was administered by gavage once a day for 8 W.Measured blood glucose,diamine oxidase(DAO),lipopolysaccharides(LPS),D-lactate contents(D-LA)in serum by enzyme linked immunosorbent assay method;Claudin-1,Occludin,Zonula Occluden-1(ZO-1)mRNA and protein expressions in lleal tissue were detected by reverse transcription-polymerase chain reaction and Western blot.Results The blood glucose in the blank group,model group,positive control group,experimental-H,-M,-L groups were(5.04±0.40),(30.71±1.21),(18.63±6.72)and(19.90±3.30)mmol·L-1;the DAO were(49.56±6.13),(192.19±24.40),(130.63±19.90)and(120.24±17.53)pg·mL-1;the LPS were(41.11±4.56),(99.67±6.63),(64.51±8.59)and(63.07±4.89)ng·L-1;the D-LA were(506.45±52.22),(1 826.49±224.17),(1 166.47±121.78)and(1 344.82±130.65)μg·L-1;the relative expression levels of Claudin-1 mRNA were 1.03±0.32,0.25±0.12,0.94±0.40 and 0.71±0.21;the relative expression levels of Occludin mRNA were 1.07±0.48,0.26±0.06,1.23±0.42 and 0.99±0.47;the relative expression levels of ZO-1 mRNA were 1.00±0.13,0.43±0.18,0.85±0.07 and 0.69±0.08;the relative expression levels of Claudin-1 protein were 1.00±0.00,0.21±0.19,0.56±0.31 and 0.87±0.31;the relative expression levels of Occludin protein were 1.01±0.27,0.38±0.11,0.88±0.10 and 0.85±0.18;the relative expression levels of ZO-1 protein were 1.00±0.14,0.43±0.04,0.77±0.02 and 0.67±0.16.Compared with the the blank group,the above indexes in the model group had statistical significance(P＜0.01,P＜0.05);compared with the model group,the above indexes in the experimental-H had statistical significance(P＜0.01,P＜0.05).Conclusion HPS can reduce intestinal mucosal injury and maintain the integrity of the intestinal mucosal barrier in spleen deficiency DGP rats.关键词
红芪多糖/脾虚/糖尿病胃轻瘫/肠黏膜屏障Key words
hedysarum polybotrys polysacchcaide/spleen deficiency/diabetic gastroparesis/intestinal mucosal barrier分类
医药卫生引用本文复制引用
魏昭晖,万生芳,李荣科,郭倩,马欣欣..红芪多糖对脾虚型DGP大鼠肠道黏膜屏障的保护作用[J].中国临床药理学杂志,2025,41(4):522-526,5.基金项目
国家自然科学基金地区基金资助项目(82060914) （82060914）
甘肃省优秀博士生基金资助项目(23JRRA1225) （23JRRA1225）
甘肃中医药大学研究生创新基金资助项目(2022-07) （2022-07）
