中国临床药理学杂志2025，Vol.41Issue(4)：527-532,6.DOI:10.13699/j.cnki.1001-6821.2025.04.016
β-蜕皮甾酮调控BMSCs自噬促进成骨分化的作用
Effects of β-ecdysterone in regulating BMSCs autophagy and promoting osteogenic differentiation
摘要
Abstract
Objective To investigate the intervention effect and mechanism of β-ecdyssterone on the osteogenic differentiation of rat bone marrow mesenchymal stem cells(BMSCs)based on the regulation of autophagy by the adenosine 5'-monophosphate kinase-activated protein(AMPK)/mammalian target of rapamycin(m TOR).Methods BMSCs cells were divided into control group(normal culture),low-,middle-and high-dose groups(intervened with 0.01,0.10 and 1.00 μmol·L-1ecdysterone).The expression of cell genes was determined by real-time quantitative polymerase chain reaction(qRT-PCR);and the relative expression level of cell proteins was determined by immunofluorescence(IF).Results The relative expression levels of alkaline phosphatase(ALP)mRNA in the control group,low-,middle and high-dose groups were 1.01±0.14,1.22±0.05,1.28±0.05,1.59±0.20;the relative protein expression levels of Runt-related transcription factor 2(RUNX2)mRNA were 1.00±0.07,1.45±0.07,2.11±0.32,4.67±1.45;the relative protein expression levels of phosphorylated AMPK protein were 0.07±0.01,0.11±0.01,0.06±0.01,0.18±0.01;and the relative protein expression levels of Sequestosome 1(p62/SQSTM1)protein were 1.72±0.02、1.67±0.02、0.94±0.01、0.04±0.01;the relative protein expression levels of p-mTOR protein were 0.66±0.01,0.40±0.01,0.42±0.01,0.04±0.01;and the relative protein expression levels of microtubule-associated protein 1 light chain 3 beta(LC3 B)protein were 0.07±0.01,0.20±0.01,0.87±0.05,1.27±0.04,respectively.There were statistically significant differences between the low-,middle-,and high-dose groups and the control group(P＜0.05,P＜0.001).Conclusionβ-ecdyssterone can regulate autophagy and promote osteogenic differentiation of BMSCs by activating the AMPK/mTOR pathway.关键词
β-蜕皮甾酮/5'-腺嘌呤核苷酸依赖的蛋白激酶/雷帕霉素靶蛋白通路/骨髓间充质干细胞/成骨分化/自噬Key words
β-ecdyssterone/adenosine 5'-monophosphate kinase-activated protein/mammalian target of rapamycin pathway/bone marrow mesenchymal stem cells/osteogenic differentiation/autophagy分类
医药卫生引用本文复制引用
汪可欣,白敏,宋冰,郭超,张延英,邢尚曼,宋文静,曹婷婷,汪永锋..β-蜕皮甾酮调控BMSCs自噬促进成骨分化的作用[J].中国临床药理学杂志,2025,41(4):527-532,6.基金项目
甘肃省科技重点研发计划基金资助项目(23YFFA0068) （23YFFA0068）
甘肃省教育厅高等学校科研基金资助项目(2023A-080) （2023A-080）
甘肃中医药大学成果转化培育基金资助项目(2023CGZH-21) （2023CGZH-21）
甘肃中医药大学创新创业基金项目 （）
甘肃省中医药管理局基金资助项目(GZKP-2023-19) （GZKP-2023-19）
