中国临床药理学杂志2025，Vol.41Issue(5)：660-664,5.DOI:10.13699/j.cnki.1001-6821.2025.05.012
阿美替尼对非小细胞肺癌细胞增殖、侵袭、上皮间质转化和干性的作用
Effects of almonertinib on proliferation,invasion,epithelial mesenchymal transformation and stemness of non-small cell lung cancer cells
摘要
Abstract
Objective To investigate the effects of almonertinib on the proliferation,invasion,epithelial-mesenchymal transition(EMT),and stemness of non-small-cell lung cancer(NSCLC)cells by regulating microRNA-22(miR-22)targeting ubiquitin-conjugating enzyme E2O(UBE2O).Methods Human lung adenocarcinoma cell line NCI-H1975 was randomly divided into control group(normal culture with no treatment),medium-dose group(4 μmol·L-1 almonertinib),inhibitor negative control(NC inhibitor)group(4 μmol·L-1 almonertinib+miR-22 inhibition negative control),miR-22 inhibitor group(4 μmol·L-1 almonertinib+miR-22 inhibition),miR-22 inhibitor+short hairpin negative control(sh-NC)group(4 μmol·L-1 almonertinib+miR-22 inhibition+short hairpin negative control),and miR-22 inhibitor+short hairpin UBE2O(sh-UBE2O)group(4 μmol·L-1 almonertinib+miR-22 inhibition+short hairpin UBE2O).The expression of miR-22 was detected by quantitative real-time polymerase chain reaction(qRT-PCR);Western blot analysis was performed to detect the expression of octamer-binding transcription factor 4(OCT4),SRY-Box transcription factor 2(SOX2),and Nanog;the cell proliferation rate was assessed using the 5-ethynyl-2-deoxyuridine(EdU)assay;and Transwell assays were conducted to evaluate cell invasion ability.Results The expression levels of miR-22 in the control group,medium-dose group were 1.00±0.16,2.07±0.34.The expression levels of OCT4 in the control group,medium-dose group,NC inhibitor group,and miR-22 inhibitor group were 1.00±0.16,0.63±0.09,0.67±0.08 and 0.94±0.12,respectively;SOX2 expression levels were 1.00±0.14,0.48±0.06,0.54±0.07 and 0.81±0.11;and Nanog expression levels were 1.00±0.18,0.45±0.08,0.52±0.11 and 0.89±0.15.The cell proliferation rates in the control group,medium-dose group,NC inhibitor group,miR-22 inhibitor group,miR-22 inhibitor+sh-NC group,and miR-22 inhibitor+sh-UBE2O group were(87.29±10.73)％,(41.65±7.82)％,(48.47±6.54)％,(79.31±10.26)％,(84.50±11.98)％and(36.82±6.79)％,respectively.The numbers of invading cells were(156.92±24.81),(72.53±9.06),(64.41±10.75),(143.27±26.19),(137.58±21.46)and(75.23±11.94),respectively.Compared between the control group and medium-dose group,NC inhibitor and miR-22 inhibitor groups,as well as miR-22 inhibitor+sh-NC and miR-22 inhibitor+sh-UBE2O groups showed statistically significant differences(all P＜0.05).Conclusion Almonertinib can inhibit the proliferation and invasion of NSCLC NCI-H1975 cells,likely by regulating miR-22 to target UBE2O.关键词
阿美替尼/微小核糖核酸-22/泛素结合酶E2O/非小细胞肺癌/增殖/侵袭/上皮间质转化/干性标志物Key words
almonertinib/microRNA-22/ubiquitin conjugating enzyme E2O/non-small cell lung cancer/proliferation/invasion/epithelial-mesenchymal transition/stemness markers分类
医药卫生引用本文复制引用
谢友琴,高振,张亮..阿美替尼对非小细胞肺癌细胞增殖、侵袭、上皮间质转化和干性的作用[J].中国临床药理学杂志,2025,41(5):660-664,5.基金项目
南通市卫生健康委员会科研基金资助项目(MS2023030) （MS2023030）
