中国临床药理学杂志2025，Vol.41Issue(5)：665-670,6.DOI:10.13699/j.cnki.1001-6821.2025.05.013
circCSPP1调控自噬影响胃癌细胞生长和转移能力的研究
Research of circCSPP1 affects the growth and metastasis ability of gastric cancer cells by regulating autophagy
摘要
Abstract
Objective To investigate the effects of circCSPP1 mediated autophagy on the proliferation and metastasis ability of gastric cancer cells.Methods The human gastric cancer cells NCI-N87 were divided into Vector group(transfected with Vector),circCSPP1 group(transfected with circCSPP1)and inhibitor group(transfected with circCSPP1 and incubated with 10 μmol·L-1chloroquine).The MKN-45 cells were divided into sh-NC group(transfected with circCSPP1 shRNA-NC),sh-circCSPP1 group(transfected with circCSPP1 shRNA)and agonist group(transfected with circCSPP1 shRNA+1 μmol·L-1sirolimus).Flow cytometry was used to detect cell apoptosis.Transwell was used to detect cell migration and invasion.Western blot were used to detect the expression levels of microtubule associated protein light chain 3-Ⅱ(LC3-Ⅱ)and p62 protein.Results In NCI-N87 cells:the apoptosis rates of Vector,circCSPP1 and inhibitor groups were(7.95±0.66)％,(3.93±0.62)％and(7.37±0.85)％;the number of cell migrations were 122.50±12.25,169.40±16.09 and 109.00±8.22;the number of cell invasions were 73.38±12.33,117.30±12.78 and 55.25±8.73;the relative expression levels of LC3-Ⅱ protein were 0.40±0.06,1.13±0.11 and 0.44±0.08;the relative expression levels of p62 protein were 1.62±0.15,0.21±0.08 and 1.43±0.16.The differences of above indexes were statistically significant between the circCSPP1 group and the inhibitor and Vector groups(all P＜0.05).In MKN-45 cells:the cell apoptosis rates of sh-NC,sh-circCSPP1 and agonist groups were(6.81±0.68)％,(12.02±0.78)％and(5.76±1.21)％;the number of cell migrations were 191.60±21.43,87.50±10.76 and 155.60±10.36;the number of cell invasions were 150.00±11.55,61.63±11.39 and 154.50±17.99;the relative expression levels of LC3-Ⅱ protein were 1.08±0.05,0.13±0.03 and 1.18±0.06;the relative expression levels of p62 protein were 0.15±0.03,1.29±0.08 and 0.19±0.04.Compared with the sh-circCSPP1 group,the differences of above indicators in the agonist and sh-NC groups were statistically significant(all P＜0.05).Conclusion circCSPP1 can improve the proliferation and metastasis ability of gastric cancer cells by inducing autophagy.关键词
氯喹/西罗莫司/circCSPP1/胃癌/自噬/增殖/转移Key words
chloroquine/sirolimus/circCSPP1/gastric cancer/autophagy/proliferation/metastasis分类
医药卫生引用本文复制引用
傅厚丰,李炳,梁振雄,王兴麟..circCSPP1调控自噬影响胃癌细胞生长和转移能力的研究[J].中国临床药理学杂志,2025,41(5):665-670,6.基金项目
海南省卫生健康行业科研基金资助项目(22A200336) （22A200336）
