中国临床药理学杂志2025，Vol.41Issue(5)：671-675,5.DOI:10.13699/j.cnki.1001-6821.2025.05.014
miR-183-5p靶向RBMS1对乳腺癌细胞自噬和迁移的影响
Effects of miR-183-5p targeting RBMS1 on autophagy and migration of breast cancer cells
摘要
Abstract
Objective To investigate the effect of microRNA-183-5p(miR-183-5p)targeting RNA-binding motif single-stranded-interacting protein 1(RBMS1)on autophagy and migration of breast cancer cells and its mechanism.Methods Human breast cancer cells MCF-7 were divided into control group,NC inhibitor group(transfected with NC inhibitor)and miR-183-5p inhibitor group(transfected with miR-183-5p inhibitor),miR-183-5p inhibitor+si-NC group(transfected with miR-183-5p inhibitor and empty vector si-NC),miR-183-5p inhibitor+si-RBMS1 group(transfected with miR-183-5p inhibitor and RBMS1 knockdown plasmid si-RBMS1).The expression levels of autophagy and phosphatidylinositol-3-kinase(PI3 K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR)pathway-related proteins in each group were detected by Western blot,and the mobility of cells in each group was detected by scratch test.Results Control group,NC inhibitor group,miR-183-5p inhibitor group,miR-183-5p inhibitor+si-NC group and miR-183-5p inhibitor+si-RBMS1 group the relative expression levels of light chain 3 Ⅱ(LC3-Ⅱ)/light chain 3 Ⅰ(LC3-Ⅰ)protein were 0.29±0.03,0.31±0.03,0.86±0.10,0.84±0.09 and 0.43±0.05,respectively;the relative expression levels of Beclin-1 protein were 0.18±0.02,0.20±0.02,0.74±0.08,0.78±0.09 and 0.35±0.04,respectively;the relative expression levels of sequestosome-1(P62)protein were 0.93±0.12,0.89±0.10,0.51±0.06,0.54±0.06 and 0.86±0.10,respectively;the 48 h cell mobility was(62.87±7.26)％,(59.23±6.89)％,(24.13±3.49)％,(26.14±4.72)％and(40.11±5.71)％,respectively;the relative expression levels of phospho-(p-)PI3K/PI3K protein were 0.67±0.12,0.64±0.10,0.32±0.06,0.35±0.06 and 0.74±0.09,respectively.The above indexes of miR-183-5p inhibitor+si-RBMS1 group were compared with those of miR-183-5p inhibitor+si-NC group,and those of miR-183-5p inhibitor group were compared with those of NC inhibitor group,and the differences were statistically significant(all P＜0.05).Conclusion Inhibition of miR-183-5p expression can promote autophagy and inhibit cell migration in MCF-7 cells,while down-regulation of RBMS1 has the opposite effect,which is related to the PI3 K/Akt/mTOR pathway.关键词
微小RNA-183-5p/乳腺癌/细胞自噬/细胞迁移/RNA结合基序单链相互作用蛋白1/磷脂酰肌醇-3-激酶/苏氨酸蛋白激酶B/雷帕霉素靶蛋白通路Key words
microRNA-183-5p/breast cancer/autophagy/cell migration/RNA-binding motif single-stranded-interacting protein 1/phosphatidylinositol-3-kinase/protein kinase B/mechanistic target of rapamycin pathway分类
医药卫生引用本文复制引用
陈登云,吴一波,黄琨波,张汉辉,张志珊,洪志鹏..miR-183-5p靶向RBMS1对乳腺癌细胞自噬和迁移的影响[J].中国临床药理学杂志,2025,41(5):671-675,5.基金项目
福建省自然科学基金资助项目(2023J011775) （2023J011775）
