中国临床药理学杂志2025，Vol.41Issue(5)：676-680,5.DOI:10.13699/j.cnki.1001-6821.2025.05.015
苦豆碱抑制膀胱癌253J细胞增殖的作用及机制研究
Inhibitory effect and mechanism of aloperin on proliferation of bladder carcinoma 253J cells
摘要
Abstract
Objective To investigate the regulatory effects of aloperin on bladder cancer cell growth and endoplasmic reticulum stress-dependent mitochondrial pathway.Methods 253J cells were randomly divided into control group,experimental-L group(25.0 μmoL·L-1 aloperin),experimental-M group(50.0 μmoL·L-1 aloperin),experimental-H group(100.0 μmoL·L-1 aloperin),4-PBA group[100.0 μmoL·L-1 aloperin+20 μmoL·L-1 4-phenylbutyric acid(4-PBA)],si-NC(transfected with si-NC+100.0 μmoL·L-1 aloperin),si-CHOP group[transfected with si-CCAAT-enhancer-binding protein homologous protein(CHOP)+100.0 μmoL·L-1 aloperin].Cell proliferation was detected by 5-ethynyl-2'-deoxyuridine(EdU)assay;protein expression was detected by Western blot assay;apoptosis was detected by flow cytometry;mitochondrial membrane potential(MMP)was detected by JC-1 assay.Results The EdU positive cell rates in control group,experimental-L group,experimental-M group and experimental-H group were(37.51±2.98)％,(29.52±2.19)％,(23.10±2.58)％and(12.94±2.52)％,respectively;the expression levels of C/EBP homologous protein(CHOP)were 0.29±0.04,0.53±0.05,0.71±0.08,0.90±0.12,respectively.The apoptosis rates in control group,experimental-H group and 4-PBA group were(4.79±0.62)％,(23.01±2.03)％and(13.75±1.14)％,respectively.The expressions of CHOP protein in control group,experimental-H,si-NC and si-CHOP groups were 0.30±0.03,0.92±0.08,0.91±0.11 and 0.20±0.02,respectively;the apoptosis rates were(4.39±0.30)％,(22.11±1.82)％,(22.65±2.40)％and(15.32±1.19)％,respectively;mitochondrial cytochrome C(Cyto-C)protein levels were 0.78±0.07,0.43±0.05,0.42±0.05 and 0.65±0.06;cytoplasmic Cyto-C protein levels were 0.51±0.06,0.91±0.10,0.88±0.05 and 0.61±0.04;MMP levels were(100.00±2.79)％,(57.49±3.34)％,(54.09±4.32)％and(69.91±6.81)％,respectively.The above indexes in the experimental-L,-M,-H groups were compared with the control group,the above indexes in the 4-PBA group were compared with the experimental-H group,and the above indexes in the si-CHOP group were compared with the si-NC group,the differences were statistically significant(all P＜0.01).Conclusion Aloperin can inhibit the proliferation of 253J cells by up-regulating the endoplasmic reticulum stress-dependent mitochondrial apoptosis pathway mediated by CHOP.关键词
苦豆碱/膀胱癌/内质网应激/线粒体凋亡/增殖Key words
aloperin/bladder cancer/endoplasmic reticulum stress/mitochondrial apoptosis/proliferation分类
医药卫生引用本文复制引用
高义胜,李敬东,何相飞,高磊,徐纪元,左世帅..苦豆碱抑制膀胱癌253J细胞增殖的作用及机制研究[J].中国临床药理学杂志,2025,41(5):676-680,5.基金项目
山东省中医药科技发展计划基金资助项目(M-2022106) （M-2022106）
