中国临床药理学杂志2025，Vol.41Issue(5)：681-685,5.DOI:10.13699/j.cnki.1001-6821.2025.05.016
他泽司他对弥漫大B细胞淋巴瘤干性和增殖的影响及其作用机制研究
Effects of tazemetostat on stem and proliferation of diffuse large B-cell lymphoma and its mechanism of action
摘要
Abstract
Objective To study the effect of tazemetostat on the stemness of diffuse large B-cell lymphoma and the related mechanism of action.Methods OCI-LY3 cells were divided into blank group,tazemetostat group and microRNA-378a-3p(miR-378a-3p)mimics group.Blank group cell conventional culture;tazemetostat group was treated with 5 μmol·L-1tazemetostat;miR-378a-3p mimics group was transfected with miR-378 a-3p mimics.The relative expression level of miR-378a-3p was detected by fluorescence in situ hybridization.mRNA expression levels of dryness related factors were detected by quantitative real time polymerase chain reaction;the expression of signal pathway-related proteins was detected by Western blot.Results The relative expression levels of miR-378a-3p in blank group and tazemetostat group were 1.00±0.14 and 0.52±0.08,respectively,the difference was statistically significant(P＜0.001).The relative expression of octamer binding transcription factor 4 mRNA in blank group,tazemetostat group and miR-378a-3p mimics group were 1.00±0.16,0.21±0.06 and 0.49±0.08,respectively;the relative expression of sex determining region Y box protein 2 mRNA were 1.00±0.09,0.39±0.05 and 0.85±0.11,respectively;the relative expression of Nanog homeobox mRNA were 1.00±0.13,0.17±0.03 and 0.65±0.10,respectively;the relative expressions of Kruppel like factor 4 mRNA were 1.00±0.10,0.28±0.05 and 0.86±0.14,respectively;the relative expressions of Yes associated protein were 1.00±0.19,3.46±0.52 and 2.14±0.36,respectively;the relative expression of transcriptional coactivator with PDZ-binding motif protein were 1.00±0.14,1.90±0.35 and 1.51±0.33,respectively.The above indexes of tazemetostat group were compared with those of blank group,and the above indexes of miR-378a-3p mimics group were compared with those of tazemetostat group,the differences were statistically significant(P＜0.001,P＜0.05).Conclusion Tazemetostat can inhibit the stemness of OCI-LY3 cells by down-regulating the level of miR-378a-3p,which may be achieved through the Yes associated protein/transcriptional coactivator with PDZ-binding motif signaling pathway.关键词
他泽司他/弥漫大B细胞淋巴瘤/细胞干性/细胞增殖Key words
tazemetostat/diffuse large B-cell lymphoma/cell stemness/cell proliferation分类
医药卫生引用本文复制引用
陈琦,彭群艺,韩妍,吴立德..他泽司他对弥漫大B细胞淋巴瘤干性和增殖的影响及其作用机制研究[J].中国临床药理学杂志,2025,41(5):681-685,5.基金项目
泉州市科技计划基金资助项目(2022NS053) （2022NS053）
