中国临床药理学杂志2025，Vol.41Issue(5)：686-690,5.DOI:10.13699/j.cnki.1001-6821.2025.05.017
毛冬青皂苷A改善高脂血症发挥心脏保护作用的研究
Study on mechanism of cardioprotective effect of Ilexin A in improving hyperlipidemia
摘要
Abstract
Objective To investigate the cardioprotective mechanism of Ilexin A in ameliorating hyperlipidemia through the regulation of microRNA-133a-3p(miR-133a-3p)and uncoupling protein 2(UCP2).Methods Rats were randomly divided into control group(gavage with an equal volume of distilled water as the experimental group),model group(gavage with an equal volume of distilled water as the experimental group),experimental group(gavage with 40 mg·kg-1 Ilexin A),miR-133a-3p inhibitor group(gavage with 40 mg·kg-1 Ilexin A+tail vein injection of 1.95 × 1012 vg·mL-1 adenovirus containing miR-133a-3p inhibitor),and OE-UCP2 group(gavage with 40 mg·kg-1 Ilexin A+tail vein injection of 1.95 × 1012 vg·mL-1 adenovirus containing both miR-133a-3p inhibitor and OE-UCP2).Blood lipid levels,including low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C),were measured by enzyme-linked immunosorbent assay.Cardiac function parameters,such as the mitral inflow peak early diastolic velocity to late diastolic velocities(E/A)and left ventricular ejection fraction(LVEF),were assessed via echocardiography.The relative expression levels of mRNA were analyzed by real-time quantitative polymerase chain reaction(RT-qPCR),while protein levels were detected by Western blot.Results In the model and experimental groups,LDL-C levels were(0.92±0.12)and(0.76±0.09)mmol·L-1;HDL-C levels were(0.82±0.10)and(1.14±0.13)mmol·L-1;E/A ratios were 0.89±0.11 and 1.17±0.14;LVEF values were(41.31±5.07)％and(59.92±7.86)％;the relative expression levels of miR-133a-3p were 0.33±0.06 and 1.22±0.18;uncoupling protein 2(UCP2)mRNA levels were 0.57±0.08 and 0.79±0.09;cleaved caspase-3 protein levels were 1.00±0.15 and 0.71±0.09;B-cell lymphoma-2(Bcl-2)protein levels were 1.00±0.17 and 1.94±0.27,respectively.The differences between the model and experimental groups for these parameters were statistically significant(all P＜0.05).Conclusion Ilexin A ameliorates hyperlipidemia by regulating miR-133a-3p and UCP2.Its mechanism may involve downregulating Bcl-2 and activating cleaved caspase-3 to induce apoptosis,thereby exerting cardioprotective effects and improving cardiac function.关键词
毛冬青皂苷A/解偶联蛋白2/高脂血症/心脏保护作用/凋亡蛋白Key words
Ilexin A/uncoupling protein 2/hyperlipidemia/cardiac protective effect/apoptotic protein分类
医药卫生引用本文复制引用
张颖,孙理华,时司宴,王维浩,彭海军..毛冬青皂苷A改善高脂血症发挥心脏保护作用的研究[J].中国临床药理学杂志,2025,41(5):686-690,5.基金项目
新疆维吾尔自治区自然科技基金资助项目(2018D01C300) （2018D01C300）
中山市社会公益科技研究基金资助项目(2023B1059) （2023B1059）
