中国临床药理学杂志2025，Vol.41Issue(5)：691-695,5.DOI:10.13699/j.cnki.1001-6821.2025.05.018
荜茇酰胺改善睡眠剥夺诱发的小鼠肝脂肪变性的作用
Effects of piperlongumine on liver steatosis induced by sleep deprivation in mice
摘要
Abstract
Objective To explore the effect and mechanism of piperlongumine on hepatic steatosis induced by sleep deprivation.Methods Mice were randomly divided into control group(normal feeding),model group(sleep deprivation treatment),low-,middle-and high dose groups(2.5,5.0 and 10.0 mg·kg-1 piperlongumine was given on the basis of sleep deprivation),and the mice were given continuous gavage for 14 days.Liver index was detected after 14 days of continuous gavage.Western blot assay was used to detect protein expression in hepatic tissue,lipid levels in hepatic tissue were detected by kit,and reactive oxygen species(ROS)levels in hepatic tissue were detected by dihydroethidium(DHE).Results The liver index of control group,model group,and high-dose group were(2.99±0.40)％,(4.32±0.17)％and(3.30±0.37)％,respectively;triglyceride(TG)levels were(0.16±0.011),(0.29±0.02)and(0.17±0.02)mmol·mgprot-1,respectively;fatty acid synthase(FAS)protein levels were 0.31±0.03,0.69±0.08 and 0.35±0.03,respectively;hypoxia-inducing factor-1 α(HIF-1 α)protein levels were 0.38±0.04,0.94±0.08 and 0.44±0.04,respectively;the protein levels of adaptor p66Shc(p66Shc)were 0.21±0.03,0.82±0.07 and 0.37±0.04,respectively;ROS levels were 1.00±0.09,4.72±0.44 and 1.30±0.07,respectively.The above indexes in the model group were significantly different from those in the control group,and the above indexes in the high-dose groups were significantly different from those in the model group(all P＜0.05).Conclusion Piperlongumine can improve liver steatosis induced by sleep deprivation in mice,which may be related to the regulation of HIF-1α/p66Shc signaling pathway.关键词
荜茇酰胺/睡眠剥夺/肝脂肪变性/缺氧诱导因子-1α/衔接蛋白p66Shc信号/线粒体功能Key words
piperlongumine/sleep deprivation/hepatic steatosis/hypoxia-inducible factor-1α/adaptor protein p66shc signal/mitochondrial function分类
医药卫生引用本文复制引用
刘滢,黄燕花,程创,熊伟,侯良绢..荜茇酰胺改善睡眠剥夺诱发的小鼠肝脂肪变性的作用[J].中国临床药理学杂志,2025,41(5):691-695,5.基金项目
重庆市教育委员会科学技术研究基金资助项目(KJQN202002711) （KJQN202002711）
