摘要
Abstract
Objective To study the effect of hydroxylsafflower yellow A(HSYA)on epiphyseal injury of tibial in rats through neurotrophic factor-3(NT-3).Methods Fifty male SD rats were randomly divided into control group,model group,HSYA group,NC group and sh-NT-3 group,with 10 rats in each group.Except the control group,the injury model of tibial epiphyseal was established in the other 4 groups by high speed dental abrasive drill.The HSYA group was given 10 mg·kg-1 HSYA by intragastric administration once a day for 6 weeks.NC group and sh-NT-3 group were respectively injected with 1 mg·kg-1 sh-NC and sh-NT-3 through the tail vein,once every two days for 6 weeks.The control group and model group were given the same amount of normal saline intragastric and caudal intravenous injection.Quantitative real-time polymerase chain reaction was used to detect the expression level of NT-3 mRNA.The expression levels of bone repair markers and Notch receptor 1(Notch1)signaling pathway related proteins were detected by Western blot.Results The mRNA levels of NT-3 in control group,model group and HSYA group were 1.00±0.17,3.31±0.59 and 5.16±0.84,respectively.The relative expression of runt related transcription factor 2 protein in control group,model group,HSYA group,NC group and sh-NT-3 group were 1.00±0.17,3.67±0.63,7.36±1.35,7.31±1.42,5.74±1.32,respectively;the relative expressions of osteoblast-specific transcription factor protein were 1.00±0.18,5.76±1.01,8.49±1.42,8.56±1.54,6.48±1.16,respectively;the relative expressions of Notch1 protein were 1.00±0.18,1.44±0.23,1.79±0.34,1.83±0.35,1.52±0.27,respectively.The above indexes in the model group were compared with the control group,the HSYA group was compared with the model group,and the sh-NT-3 group was compared with the NC group,and the above indexes were statistically significant(all P＜0.05).Conclusion HSYA improves tibial epiphyseal injury in rats by promoting NT-3 expression and activating Notch1 signaling pathway.关键词
羟基红花黄色素A/神经营养因子-3/骨骺损伤/Notch1信号通路/胫骨Key words
hydroxysafflower yellow A/neurotrophin-3/epiphyseal injury/Notch1 signaling pathway/tibial bone分类
医药卫生
