摘要
Abstract
Objective To study the effect and mechanism of galangin in improving depression-like behavior in vascular dementia(VD)mice through microRNA-134(miRNA-134).Methods Seventy C57BL/6J mice were randomly divided into sham group(0.9％NaCl),model group(0.9％NaCl),low-dose group(25 mg·kg-1 galangin),medium-dose group(50 mg·kg-1 galangin),high-dose group(100 mg·kg-1 galangin),and mimic NC group(100 mg·kg-1 galangin and mimic NC 15 nmol),miRNA-134 mimic group(100 mg·kg-1 galangin and miRNA-134 mimic 15 nmol).The degree of depression was detected by tail suspension test and forced swimming test;the level of corticosterone was detected by enzyme-linked immunosorbent assay;the level of miRNA-134 was detected by quantitative real time polymerase chain reaction;the apoptosis rate of brain tissue cells was detected by TdT-mediated dUTP nick-end labeling;and the level of synaptic plasticity related protein was detected by Western blot.Results The suspension time of the sham group,the model group and the high-dose group were(105.32±15.22),(185.32±24.62)and(112.65±15.08)s,respectively;the swimming time were(86.52±13.25),(152.32±22.22)and(96.22±16.35)s respectively;the levels of corticosterone were(32.65±5.02),(86.62±14.32)and(48.52±5.55)ng·L-1,respectively;the levels of miRNA-134 were 1.00±0.12,2.32±0.35 and 1.21±0.18,respectively.The apoptosis rates of the sham group,model group,high group,mimic NC group and miRNA-134 mimic group were(5.32±0.62)％,(62.33±8.65)％,(14.32±1.78)％,(15.85±2.22)％and(43.21±7.05)％,respectively;synaptophysin protein levels were 1.00±0.18,0.32±0.05,0.88±0.12,0.82±0.11 and 0.30±0.05,respectively;the postsynaptic density protein-95 protein levels were 1.00±0.14,0.61±0.07,0.72±0.09,0.70±0.08 and 0.45±0.06,respectively.The above indexes in the model group were compared with those in the sham group,those in the high-dose group were compared with those in the model group,and those in the miRNA-134 mimic group were compared with those in the mimic NC group,and the differences were statistically significant(all P＜0.05).Conclusion Galangin may improve the depression-like behavior of VD mice by inhibiting the expression of miRNA-134,improving synaptic plasticity and inhibiting cell apoptosis.关键词
高良姜素/神经突触/血管性痴呆/抑郁/微小RNA-134Key words
galangin/synapses/vascular dementia/depression/microRNA-134分类
医药卫生
