中国免疫学杂志2025，Vol.41Issue(4)：867-872,6.DOI:10.3969/j.issn.1000-484X.2025.04.016
胰高血糖素样肽-2对TNBS诱导的克罗恩病大鼠肠黏膜组织损伤及Th1/Th2细胞免疫平衡的影响
Effects of glucagon-like peptide-2 on TNBS-induced intestinal mucosal tissue injury and Th1/Th2 cell immune balance in Crohn's disease
摘要
Abstract
Objective:To investigate the effect of glucagon like peptide-2(GLP-2)on 2,4,6-trinitrobenzenesulfonic acid(TNBS)induced intestinal mucosal tissue damage and the effects of helper T cell 1/helper T cell 2(Th1/Th2)balance in rats with Crohn's disease(CD).Methods:Forty rats were randomly divided into control group,CD group,treatment(CD+GLP-2)group and positive control[CD+sulfasalazine(SASP)]group,with 10 rats in each group.TNBS induced CD model was established and the rats in the other 3 groups were given corresponding treatment.After completion,colon macroscopic damage index(CMDI)was evaluated,HE staining was used to observe the histopathological changes of intestinal mucosa,TUNEL staining was used to detect apoptosis of co-lonic tissue cells,ELISA was used to determine serum IFN-γ,TNF-α,IL-12,IL-4 and IL-10,immunohistochemical staining was used to detect the expressions of IFN-γ and IL-4 in colon tissues,flow cytometry was used to determine the proportion of Th1/Th2 in spleen.Results:Compared with CD group,CMDI in CD+GLP-2 group and CD+SASP group were significantly decreased(P all<0.001),intes-tinal mucosal damage was improved,the positive rate of TUNEL in colon tissue was significantly decreased(P all<0.001),the con-tents of IFN-γ,TNF-α and IL-12 in serum were significantly decreased(P all<0.001),the contents of IL-4 and IL-10 in serum were significantly increased(P all<0.001),the expression of IFN-γ in colon tissue was significantly decreased and the expression of IL-4 was significantly increased(P all<0.001),the proportion of Th1 cells was significantly decreased while the proportion of Th2 cells was significantly increased(P all<0.001),Th1/Th2 also decreased significantly(P all<0.001).Conclusion:GLP-2 can effectively im-prove the intestinal mucosal injury of CD rats induced by TNBS,and the mechanism may be related to regulating the immune balance of Th1/Th2 cells,inhibiting inflammatory response and reducing cell apoptosis.关键词
克罗恩病/胰高血糖素样肽-2/肠黏膜损伤/辅助性T细胞1/辅助性T细胞2平衡Key words
Crohn's disease/Glucagon-like peptide-2/Intestinal mucosal injury/Helper T cell 1/helper T cell 2 balance分类
医药卫生引用本文复制引用
陈卿奇,庞莉,冯彩团,李文妍,程芃..胰高血糖素样肽-2对TNBS诱导的克罗恩病大鼠肠黏膜组织损伤及Th1/Th2细胞免疫平衡的影响[J].中国免疫学杂志,2025,41(4):867-872,6.基金项目
海南省基础与应用基础研究计划(省自然科学基金)立项项目(821RC733) （省自然科学基金）
海南省卫生健康行业科研项目(20A200397). （20A200397）
