中国免疫学杂志2025，Vol.41Issue(4)：873-878,6.DOI:10.3969/j.issn.1000-484X.2025.04.017
血红素氧化酶1调控自噬对脓肿分枝杆菌胞内生长的影响
Effects of heme oxygenase-1 on autophagy regulation and intracellular growth of Mycobacterium abscessus
摘要
Abstract
Objective:To investigate the role of heme oxygenase-1(HO-1)regulating autophagy and its impact on the intracel-lular growth of Mycobacterium abscessus(M.abs).Methods:The standard strain of M.abs ATCC19977 was used to stimulate mouse RAW264.7 macrophages with a specified multiple of infection(MOI)for a specified time.Western blot analysis was performed to detect the expression levels of HO-1 protein and autophagy related proteins Atg5,LC3Ⅱ and p62.Macrophages were pretreated with HO-1 specific inducer CoPP and enzyme activity inhibitor SnPP for 12 h,and incubated with M.abs for 2 h.Amikacin removed extra-cellular bacteria and continued to culture until the specified time,and cell activity was detected by CCK-8 method.Western blot and LysoTracker Red were used to detect the regulatory relationship between HO-1 protein and autophagy related proteins.Bacterial survival and TNF-α secretion levels were detected by colony count and ELISA.Results:Compared with the control group,M.abs could induce increased expression of HO-1,Atg5,LC3Ⅱ and p62 proteins.Over-expression and inhibition of HO-1 were found to effectively regu-late the expressions of Atg5,LC3Ⅱ and p62 induced by M.abs.LysoTracker Red,colony count,and ELISA results demonstrated that SnPP promoted the increasement of intracellular lysosomes,significantly inhibited M.abs intracellular proliferation,and reduced TNF-α secretion.Conclusion:Inhibition of HO-1 can enhance the autophagy flow induced by M.abs and reduce the intracellular growth of M.abs,which provides scientific basis for the development of targeted drugs targeting HO-1.关键词
脓肿分枝杆菌/血红素氧化酶1/自噬/菌落计数Key words
Mycobacterium abscessus/Heme oxygenase-1/Autophagy/Colony count分类
医药卫生引用本文复制引用
刘爱凤,罗海军,谢仁峰,马小华..血红素氧化酶1调控自噬对脓肿分枝杆菌胞内生长的影响[J].中国免疫学杂志,2025,41(4):873-878,6.基金项目
湖南省卫生健康委课题(20201287) （20201287）
湖南省自然科学基金项目(2021JJ70130,2025JJ80543). （2021JJ70130,2025JJ80543）
