摘要
Abstract
Objective:To explore the prognostic model for construction and preliminary evaluation of tumor invasion of im-mune cells after hemithyroidectomy in patients with thyroid cancer.Methods:The gene expression profiles and follow-up parameters of 152 patients with thyroid cancer after hemithyroidectomy from November 2020 to November 2022 were selected from The Cancer Genome Atlas(TCGA);ssGSEA was used to quantify immune cell infiltration in tumor tissues.LASSO was used to screen for key pre-dictors to verify the relationship between prognosis and different infiltrating immune cells.A prognostic risk score model was constructed using appropriate immune cells,and thyroid cancer patients were stratified into high-and low-risk groups.Kaplan-Meier survival curves were used for validation.Nomogram models were developed based on the risk model to predict survival rates and treatment inef-ficiency in thyroid cancer patients.Model accuracy was verified using ROC curve and AUC value.Calibration curves were employed to compare predicted and observed outcomes,and clinical decision curves were used to assess model reliability.Results:Immune cell in-filtration data were quantified by ssGSEA to obtain 22 infiltrating immune cells,including activated mast cells,naive B cells,unacti-vated mast cells,plasma cells,CD8+T cells,γδT cells,naive CD4+T cells,activated memory CD4+T cells,Treg cells,follicular helper T cells,memory B cells,activated NK cells,unactivated NK cells,unactivated memory CD4+T cells,M0 macrophages,monocytes,M1 macrophages,neutrophils,unactivated dendritic cells,activated dendritic cells,M2 macrophages and eosinophils.Six immune cells were selected as predictors,M0 macrophages,M2 macrophages,activated dendritic cells,unactivated mast cells,CD8+T cells,and monocytes.Risk scores calculated from these six immune cells were positively correlated with poor prognosis in high-risk patients.Kaplan-Meier analysis showed significantly longer overall survival in the low-risk group compared to the high-risk group(Log-Rank χ2=4.524,P=0.024).The total score of the prognostic nomogram model was 425,and the mortality risk was 79.12%;the treatment efficacy nomogram model had a total score of 406 points,corresponding to a 77.97%risk of treatment failure.Model validation results were reliable.Conclusion:The nomogram model of immune cells infiltration in thyroid cancer patients suggest that immune cell infiltration can be used as an important indicator to predict therapeutic efficacy and prognostic outcome in thyroid cancer patients.关键词
免疫细胞浸润/预后模型/甲状腺癌/治疗与预后Key words
Immune cell infiltration/Prognostic model/Thyroid cancer/Treatment and prognosis分类
医药卫生
