摘要
Abstract
Objective:To investigate the relationship between the changes of immune microenvironment and prognosis in naso-pharyngeal carcinoma patients undergoing concurrent radiotherapy and chemotherapy.Methods:A total of 134 patients with nasopha-ryngeal carcinoma who were treated with concurrent radiotherapy and chemotherapy were selected to analyze the expression levels of six immune cell markers in tumor tissue microarray by immunohistochemistry.They were divided into high expression group and low expression group,and the correlation between six kinds of immunohistochemical indicators and the clinical pathological characteristics of patients were analyzed.According to the prognosis of patients,they were divided into poor prognosis group(n=41)and good progno-sis group(n=93).Multifactor analysis was conducted through Logistic regression model to construct nomogram prediction model and verify the model.Kaplan-Meier method and Log-rank test were used to analyze the influence of the expression levels of six immunohis-tochemical indicators on the five-year survival rate of patients.Results:After concurrent radiotherapy and chemotherapy,the expres-sion levels of CD3,CD4,CD8 and CD56 in tumor tissues of patients were increased significantly,and the average positive rates of CD10 and FOXP3 were significantly lower than those before treatment(P<0.05).The expression levels of CD3,CD4,CD8 and CD56 were negatively correlated with tumor size and lymph node metastasis(P<0.05);the expression levels of CD10 and FOXP3 were posi-tively correlated with tumor size and lymph node metastasis(P<0.05).Tumor size,lymph node metastasis,CD10 and FOXP3 were in-dependent risk factors affecting the prognosis of patients with nasopharyngeal carcinoma(P<0.05),while CD3,CD4,CD8 and CD56 were protective factors(P<0.05).Patients with higher expression levels of CD3,CD4,CD8 and CD56 and lower expression levels of CD10 and FOXP3 have higher five-year survival rates.Conclusion:After concurrent radiotherapy and chemotherapy,the average posi-tive rates of CD3,CD4,CD8,CD56 are significantly increased compared with those before treatment,and the average positive rates of CD10 and FOXP3 are significantly decreased compared with those before treatment,the prediction model based on the clinical char-acteristics of patients can effectively predict the prognosis of patients with nasopharyngeal carcinoma.关键词
鼻咽癌/放疗/化疗/免疫细胞/肿瘤微环境/预后Key words
Nasopharyngeal carcinoma/Radiotherapy/Chemotherapy/Immune cells/Tumor microenvironment/Prognosis分类
医药卫生
