中国农业大学学报2025，Vol.30Issue(5)：72-82,11.DOI:10.11841/j.issn.1007-4333.2025.05.06
秸秆源木霉黄腐酸的生物活性成分及其对白菜的促生效果
Analysis of the bioactive components of straw-derived Trichoderma fulvic acid and its growth-promoting effect on Chinese cabbage
摘要
Abstract
To identify the main bioactive substances in straw-derived Trichoderma fulvic acid(TFA)and their growth-promoting effects on Chinese cabbage,the non-targeted metabolomics analysis of TFA was conducted using liquid chromatography-tandem mass spectrometry(LC-MS).The main metabolite components and metabolic pathways in TFA before and after fermentation were analyzed.A randomized block design was employed for a field experiment with three treatments including TFA application rates of 0(CK),180(T1),and 720 mg/m2(T2),respectively.After application of TFA,indicators such as yield,fresh weight per plant,chlorophyll content(SPAD),Vc content,soluble protein and nitrate content were measured to evaluate the effects.The results showed that:A total of 903 significantly different metabolites were identified in TFA before and after fermentation.Among them,the expression levels of 58 metabolites,primarily lipids and organic acids,such as etiocholanolone glucuronide,indole-3-acetic-acid-O-glucuronide,7-ketolithocholic acid,1-methylhistidine,arginyl-tryptophan,L-glutamine,were significantly increased.The results of KEGG pathway enrichment revealed that these metabolites were distributed across 55 pathways.High-expression metabolites significantly enriched in arginine and proline metabolism,niacin and nicotinamide metabolism,and linoleic acid metabolism.The results of the field experiment showed that compared to CK,SPAD,Vc content,soluble protein content and the fresh weight per plant of Chinese cabbage in T2 increased by 43.5％,99.7％,37.1％and 29.0％,respectively,while nitrate content decreased by 23.4％.In summary,lipids and organic acids are the key metabolites in TFA with potential plant growth-promoting activity.Application of TFA as a basal fertilizer significantly improved both the yield and quality of Chinese cabbage,and the effect of T2 is better than that of T1.关键词
黄腐酸/代谢组学/木霉/白菜/促生Key words
fulvic acid/metabolomics/Trichoderma/Chinese cabbage/growth promotion分类
农业科技引用本文复制引用
孙振华,师杨杰,龙玉娇,靳红梅,朱宁..秸秆源木霉黄腐酸的生物活性成分及其对白菜的促生效果[J].中国农业大学学报,2025,30(5):72-82,11.基金项目
江苏省重点研发计划(BE2022788) （BE2022788）
江苏省自然科学基金(BK20242006) （BK20242006）
