中国农业大学学报2025，Vol.30Issue(5)：107-121,15.DOI:10.11841/j.issn.1007-4333.2025.05.09
低温胁迫对设施草莓花器形态发育及花粉活力的影响
Effects of low-temperature stress on the morphological development of facility-grown strawberry's floral organ and pollen viability
摘要
Abstract
To investigate the effects of low temperature on the morphological development of facility-grown strawberry's floral organs and pollen vitality,two strawberry varieties,the long-day variety'Selva'and the short-day variety'Toyonoka'were used as study materials.Dynamic low-temperature treatments were conducted during the strawberry budding stage,with two temperature settings(20℃ for daytime maximum and 10℃ for nighttime minimum,and 15 ℃ for daytime maximum and 5℃ for nighttime minimum)and three different exposure durations(3,6,and 9 days).The control group(CK)was maintained at 25 ℃ for daytime maximum and 15℃ for nighttime minimum.The phenotypic characteristics of floral organs,anatomical structures of sepals and petals,pollen vitality,and fruit set rate under different treatments were investigated.The results showed that:1)Low-temperature treatments delayed flowering and reduced flower opening.After 9 days of the treatment with 15℃ daytime maximum and 5℃ nighttime minimum,the maximum flower opening in'Selva'and'Toyonoka'decreased by 30.04％and 30.93％,respectively,compared to the control,and the flowering delayed by 11 days and 13 days,respectively;2)Low temperature had a significant impact on the morphological traits of floral organs.The length and width of pistil,anther,and filament in'Selva'decreased by 30.16％,33.54％,37.10％,and 48.96％,respectively,while in'Toyonoka',the reductions were 31.56％,33.56％,37.13％,and 48.16％,respectively.The length and width of sepal and petal also decreased with increasing low-temperature stress;3)After low-temperature treatment,the thickness of the epidermal cells and the overall thickness of sepals and petals in both varieties increased significantly.After 9 days of the treatment with 15℃ daytime maximum and 5℃nighttime minimum,the sepal thickness and petal thickness in'Selva'increased by 26.50％and 33.89％,respectively,while in'Toyonoka',they increased by 24.21％and 46.04％,respectively.4)With the intensifying of the low-temperature stress,the pollen vitality in both varieties significantly decreased.After 9 days of the treatment with 15℃ daytime maximum and 5℃ nighttime minimum,the pollen staining rate in'Selva'and'Toyonoka'decreased by 57.04％and 67.19％,and the pollen germination rate decreased by 56.70％and 63.46％,respectively.5)After low-temperature treatments,the fruit set rate of both varieties.decreased significantly.In conclusion,low-temperature stress had a smaller effect on the morphological development of floral organs and pollen vitality in the long-day variety'Selva'compared to the short-day variety'Toyonoka'.This study provides valuable information for temperature management during the flowering period and low-temperature disaster prevention in facility-grown strawberries.关键词
设施草莓/低温胁迫/花器官/开花进程/形态发育/解剖结构/花粉活力Key words
facility strawberry/low-temperature stress/floral organ/flowering process/morphological development/anatomical structure/pollen viability分类
农业科技引用本文复制引用
张呈静,杨再强,张涵奇,姜楠,罗靖..低温胁迫对设施草莓花器形态发育及花粉活力的影响[J].中国农业大学学报,2025,30(5):107-121,15.基金项目
国家自然科学基金面上项目(42275200) （42275200）
江苏省研究生科研与实践创新计划项目(KYCX24_1446) （KYCX24_1446）
