中国农业大学学报2025，Vol.30Issue(5)：122-129,8.DOI:10.11841/j.issn.1007-4333.2025.05.10
萱草为母本的非整倍体育种
Aneuploid breeding with Hemerocallis fulva as maternal parent
摘要
Abstract
In order to enrich the genetic variation of Hemerocallis plants,Hemerocallis fulva(2n=3x=33)was crossed with five diploid and ten tetraploid daylily varieties.Early embryo rescue was performed on the failed fruits,and the chromosome karyotypes of the F1 generation were analyzed to cultivate aneuploid daylily lines.The results showed that:1)The defeat is serious when H.fulva is used as the maternal parent.The fruiting rate of different genotypes is different,and the highest is 51.43％and the lowest is 5.99％.2)The embryo age of 10 d is suitable for inoculation of intact ovules,the average survival rate is 3.88％under embryo rescue;At the embryo age of 10 to 16 d,the cut ovules inoculation survival rate is high,the survival rate is 50.46％;When the embryo age is more than 16 d,embryo inoculation is suitable,and the survival rate is 30.95％.3)The F,generation chromosome number is 22 to 33 of H.fulva crossed with diploid varieties,and the F,generation chromosome number is 32 to 44 of it crossed with tetraploid varieties.The karyotypic analysis shows that the F,generation is aneuploid,the chromosome of the deletion and the increase of the absence and the lack of obvious pattern,also did not show obvious grouping.In conclusion,using H.fulva as the maternal parent can efficiently produce aneuploid progeny.This study,provides new methods and avenues for broadening the variation range of daylilies in future breeding efforts.关键词
萱草/非整倍体/胚拯救/核型分析Key words
daylily/aneuploid/embryo rescue/karyotyping分类
农业科技引用本文复制引用
王子仪,周静,高亦珂,王营,崔毓萱,吕奕,张启翔..萱草为母本的非整倍体育种[J].中国农业大学学报,2025,30(5):122-129,8.基金项目
国家自然科学基金项目(32371943) （32371943）
北方特色抗逆高生态效益花卉(草)新品种选育及示范项目(2022XAGG0100) （草）
