中国兽医科学2025，Vol.55Issue(4)：500-506,7.DOI:10.16656/j.issn.1673-4696.2025.0047
三穗鸭源禽致病性大肠杆菌与鸭疫里氏杆菌的分离与鉴定
Isolation and identification of avian pathogenic Escherichia coli and Riemerella anatipestifer from Sansui ducks
摘要
Abstract
Pathogens and their biological characteristics were investigated in diseased ducks from a duck farm in Sansui.Bacterial pathogens isolation and identification from pathological tissues was performed,and virulence gene detection,serotype identification,drug sensitivity testing and animal toxicity tests were conducted.The results of isolation,identification,and gene sequencing indicated that two pathogens were GZE6 and GZR6,and GZE6 was avian pathogenic Escherichia coli(APEC),carrying eight virulence genes such as yijP and irp2,which belongs to type O18 of APEC,and GZR6 was Riemerella anatipestifer(RA),carrying six virulence genes including camp and fur,which belongs to type 2 of RA.GZE6 and GZR6 exhibited resistance to aminoglycosides,macrolides and other antibiotics.Animal toxi-city tests indicated that ducklings intramuscular inoculated with GZE6 and GZR6 displayed clinical symptoms after 24 h,and all succumbed after 72 h.These findings elucidated that the diseased ducks from Sansui were affected by a mixed infection of APEC and RA,and both GZE6 and GZR6 exhibited certain levels of drug resistance and harbored multiple virulence genes and had strong pathogenicity,offering a reference for vaccine selection and antimicrobial control of pathogenic E.coli and R.anatipestifer in Sansui ducks.关键词
禽致病性大肠杆菌/鸭疫里氏杆菌/血清型/耐药性/毒力基因Key words
avian pathogenic Escherichia coli/Riemerella anatipestifer/serotype/drug resis-tance/virulence gene分类
农业科技引用本文复制引用
陈泽辉,王丽娟,姚碧琼,嵇辛勤,阮涌,曹伟,安而立,龙丹丹,陈佳琪,杨纯培,吴宗豪..三穗鸭源禽致病性大肠杆菌与鸭疫里氏杆菌的分离与鉴定[J].中国兽医科学,2025,55(4):500-506,7.基金项目
贵州省科技支撑计划项目(黔科合支撑[2022]一般137) （黔科合支撑[2022]一般137）
