中国兽医科学2025，Vol.55Issue(4)：507-511,5.DOI:10.16656/j.issn.1673-4696.2025.0062
西藏地区藏羊隐孢子虫的分子流行病学调查
Molecular epidemiological investigation of Cryptosporidium in Tibetan sheep in Tibet
摘要
Abstract
To investigate the prevalence of Cryptosporidium infection in Tibetan sheep in high-altitude regions of Tibet,420 fresh fecal samples from Tibetan sheep were collected from intensive breeding farms in Gangba County,Shigatse City,Tibet,and Cryptosporidium infection was detected using nested PCR method to amplify the SSU rRNA gene;subtype analysis of the identified Cryptosporidium was conducted through analyzing the gp60 gene.The results showed that the total infection rate of Cryp-tosporidium in Tibetan sheep was 9.0％(38/420).SSU rRNA gene analysis revealed that the Cryptosporid-ium species infecting Tibetan sheep are Cryptosporidium xiaoi and Cryptosporidium ubiquitum.Statis-tical analysis revealed significant differences in the infection rates among different age groups and genders(P＜0.05).Analysis of the gp60 gene showed that among the samples infected with C.xiaoi(n=22),18 samples were successfully classified into with 4 subtype families,including ⅩⅩⅢh(n=8),ⅩⅩⅢl(n=6),ⅩⅩⅢe(n=2),and ⅩⅩⅢd(n=2),whereas 16 samples with C.ubiquitum infection were all found to be the subtype Ⅻa(n=16).As Ⅻa subtype can infect humans and various other hosts,this data suggests a potential risk of zoonotic transmission of Cryptosporidium in sheep in this area,and effective control measures should be taken into consideration for this region.关键词
藏羊/隐孢子虫/基因亚型/人兽共患Key words
Tibetan sheep/Cryptosporidium/genetic subtypes/zoonotic分类
畜牧业引用本文复制引用
孔皓月,张月玥,马平平,牟文杰,邹扬,郭亚琼,王帅,张少华..西藏地区藏羊隐孢子虫的分子流行病学调查[J].中国兽医科学,2025,55(4):507-511,5.基金项目
国家重点研发计划项目(2022YFD1302102-06) （2022YFD1302102-06）
甘肃省科技重大专项(22ZD6NA001) （22ZD6NA001）
