中国兽医科学2025，Vol.55Issue(4)：520-527,8.DOI:10.16656/j.issn.1673-4696.2025.0057
CHO细胞表达基因工程抗体的研究进展
Research progress of genetically engineered antibodies expressed in CHO cells
摘要
Abstract
Genetically engineered antibodies have provided a new therapeutic approach for dis-eases such as tumors,autoimmune diseases and infectious diseases,having a significant clinical appli-cation value.These antibodies,a third-generation antibody,are produced through antibody gene modifi-cation,DNA recombination and protein expression techniques in suitable cells where transcription and translation take place.They possess advantages such as strong plasticity,short preparation cycle,simple operation and lower cost.At present,most approved therapeutic antibodies are produced by Chi-nese hamster ovary cells(CHO),which provide complex post-translational modifications to ensure the activity and stability of antibody drugs.In the process of developing antibody drugs,existing produc-tion platforms still face the challenges,low antibody yields,inconsistent quality and stability and high production costs.The types of genetically engineered antibodies,CHO cells and the design strate-gies of recombinant expression vector for the aim of optimizing the expression system are reviewed,serving as references for higher expression level of antibodies and lower production costs,and meeting the growing demands of the future biopharmaceutical industry.关键词
基因工程抗体/CHO细胞/重组表达载体/生产力Key words
genetically engineered antibody/CHO cell/expression vector/productivity分类
农业科技引用本文复制引用
邓思棋,朱元源,徐璐,李芳韬,赵俊杰,刘业兵,邬静,邹兴启..CHO细胞表达基因工程抗体的研究进展[J].中国兽医科学,2025,55(4):520-527,8.基金项目
国家重点研发计划项目(2021YFD1800105) （2021YFD1800105）
