中国兽医科学2025，Vol.55Issue(4)：528-537,10.DOI:10.16656/j.issn.1673-4696.2025.0065
黄芩苷对感染H9N2亚型禽流感的小鼠肺泡上皮细胞分泌Ⅰ型干扰素的影响
Effect of baicalin on the secretion of type Ⅰ interferons by alveolar epithelial cells in mice infected with H9N2 subtype avian influenza virus
摘要
Abstract
The aim of this study was to investigate the effect of baicalin on the expression of typeⅠ interferon(IFN-Ⅰ)in the alveolar epithelial cells(MLE-12 cells)of mice infected with H9N2 avian influenza virus(H9N2 AIV),and further reveal the mechanism of baicalin's anti-avian influenza viral effects in vitro,as well as the relationship between baicalin and the immune function of the MLE-12 cells,with a view to providing theoretical basis for the antiviral mechanism of traditional Chinese medicine.A model of MLE-12 cells infected with H9N2 AIV was established in vitro,and the MOI of MLE-12 cells by H9N2 AIV was determined by plaque method,and the safe dose of baicalin was determined by toxicity assay,and the expression of IFN-Ⅰ in MLE-12 cells was detected by fluorescence quantitative PCR and ELISA.The MOI of MLE-12 cells infected by H9N2 AIV was 0.05,and the safe concentration of baicalein was less than 20 μg/mL.H9N2 AIV was able to induce the expression of IFN-α and IFN-β in MLE-12 cells.Its relative expression of IFN-α and IFN-β genes was significantly increased in baicalein high-dose group(15 μg/mL)at 24 h(P＜0.05).The protein expression of IFN-α in MLE-12 cells was signficantly increased in the low,medium,and high dose groups of baicalin at 6 h(P＜0.05),and the protein expression of IFN-β in MLE-12 cells was significantly increased at 12 and 24 h of action(P＜0.05).H9N2 AIV induced the expression of IFN-αand IFN-β in MLE-12 cells.Baicalein could induce the expression of IFN-α and IFN-β in MLE-12 cells.Baicalin rapidly responds to H9N2 AIV infection by increasing the expression level of IFN-β in MLE-12 cells to exert antiviral effect.关键词
黄芩苷/H9N2亚型禽流感病毒/小鼠肺泡上皮细胞/Ⅰ型干扰素Key words
baicalin/H9N2 subtype avian influenza virus/mouse alveolar epithelial cells/type Ⅰinterferon分类
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梁丽娜,孙红丹,乌仁图亚,张涛,胡格..黄芩苷对感染H9N2亚型禽流感的小鼠肺泡上皮细胞分泌Ⅰ型干扰素的影响[J].中国兽医科学,2025,55(4):528-537,10.基金项目
国家自然科学基金项目(30500368) （30500368）
