中国兽医杂志2025，Vol.61Issue(4)：28-36,9.DOI:10.20157/j.cnki.zgsyzz.2025.04.004
西藏自治区部分地区家犬细粒棘球绦虫和十二指肠贾第虫感染情况调查
Investigation on Echinococcus granulosus and Giardia duodenalis Infections in Domestic Dogs in Certain Areas of the Tibet Autonomous Region
摘要
Abstract
To understand the prevalence of Echinococcus granulosus(E.granulosus)and Giardia duodenalis(G.duodenalis)infections in domestic dogs in certain areas of Tibet,this study collected 553 fecal samples from domestic dogs in four townships(Gongka Town,Riduo Township,Niangpu Township,and Jiaxing Township)in Lhasa and Nyingchi.Nested PCR targeting the nicotinamide dehydrogenase subunit Ⅰ(nad Ⅰ)gene of E.granulosus and the triose phosphate isomerase(tpi),beta-giardins(bg),and glutamate dehydrogenase(gdh)genes of G.duodenalis was performed to detect infections.PCR-positive products were further sequenced,aligned,and subjected to phylogenetic analysis to determine the genetic relationships and evolutionary status of the detected parasites.The results showed that 57 samples tested positive for E.granulosus,with an overall prevalence of 10.3%(57/553).Phylogenetic analysis indicated that the positive samples belonged to the G5 genotype(Echinococcus ortleppi).Additionally,46 samples tested positive for G.duodenalis,with a prevalence of 8.3%(46/553).Phylogenetic analysis revealed that the positive G.duodenalis samples belonged to assemblages C,D,and G.These findings indicate a high prevalence of E.granulosus and G.duodenalis infections in domestic dogs in certain areas of Tibet.The E.granulosus G5 genotype detected in dog feces has been rarely reported in China,while the G.duodenalis assemblage G detected only infects rodents,suggestting a new epidemiological pattern of these parasites in the surveyed areas.This study provides important data support for the prevention and control of dog's parasitic diseases in Tibet.Future surveillance efforts should focus on strengthening monitoring of dogs,improving management practices,and implementing effective deworming programs.关键词
家犬/细粒棘球绦虫/十二指肠贾第虫/巢式PCR/G5基因型/集聚体GKey words
domestic dog/Echinococcus granulosus/Giardia duodenalis/nested PCR/G5 genotype/assemblage G分类
畜牧业引用本文复制引用
杨涛,普布卓玛,宁立宏,冯青,郝力力,拉巴次仁,党发发,旺扎,益西尊姆,向阳,次仁桑珠,白玛玉珍..西藏自治区部分地区家犬细粒棘球绦虫和十二指肠贾第虫感染情况调查[J].中国兽医杂志,2025,61(4):28-36,9.基金项目
中央引导地方项目(XZ202202YD0032C) （XZ202202YD0032C）
