中国兽医杂志2025，Vol.61Issue(4)：44-52,9.DOI:10.20157/j.cnki.zgsyzz.2025.04.006
瑞香狼毒对小鼠肝脏损伤和糖代谢的影响
Effect of Stellera chamaejasme L.on Liver Injury and Glucose Metabolism in Mice
摘要
Abstract
To investigate the effects of the toxic plant Stellera chamaejasme L.on liver injury and glucose metabolism in mice,this study divided the mice into three groups:Negative control group,Experimental feeding I group,and Experimental feeding II group,which were fed 0,5,and 10 g/(kg·d)of Stellera chamaejasme L.,respectively.Clinical symptoms were observed,and body weight gain was measured.Serum biochemical parameters were analyzed using enzyme linked immunosorbent assay(ELISA),and liver histopathological changes were examined through hematoxylin-eosin(H.E.)staining.Quantitative real-time polymerase chain reaction(qPCR)was employed to assess the expression levels of glucose metabolism-related genes,including AMP-activated protein kinase α1(Ampkα1),signal transducer and activator of transcription 3(Stat3),glucose-6-phosphatase catalytic subunit 1(G6pc1),and sirtuin 1(Sirt1)in mouse liver.Western blot(WB)analysis was conducted to detect AMPKα1 protein expression in liver tissues.The results showed that both experimental groups exhibited varying degrees of depression,slow movement,and unkempt fur.Compared with the Negative control group,body weight gain in Experimental feeding I group was significantly reduced on days 7 and 28(P<0.05 or P<0.01),while in Experimental feeding II group,body weight gain was significantly reduced throughout the experiment(P<0.001).Additionally,body weight gain in Experimental feeding II group was significantly lower than in Experimental feeding I group on days 14,21,and 28(P<0.01 or P<0.001).Compared with the Negative control group,both experimental groups showed significantly reduced glucose(GLU)and total protein(TP)levels(P<0.001)and significantly increased alkaline phosphatase(ALP)and alanine aminotransferase(ALT)activities(P<0.001).In Experimental feeding II group,total cholesterol(TCH),total bilirubin(TBIL)levels,and aspartate aminotransferase(AST)activity were also significantly elevated(P<0.001).Compared with Experimental feeding I group,Experimental feeding II group exhibited significantly lower GLU levels(P<0.01)and significantly higher TCH,TBIL levels,and ALP,ALT,AST activities(P<0.01 or P<0.001).Histopathological examination revealed that both experimental groups exhibited structural disruption of hepatic cords,hepatocyte necrosis,congestion,and inflammatory cell infiltration.Compared with the Negative control group,Sirt1 and G6pc1 gene expression levels were significantly downregulated in Experimental feeding I group(P<0.05),while Ampkα1,Stat3,and G6pc1 expression levels were significantly downregulated in Experimental feeding II group(P<0.01 or P<0.001).The expression levels of Ampkα 1,Sirt1,Stat3,and G6pc1 genes in Experimental feeding II group were significantly lower than in Experimental feeding I group(P<0.05,P<0.01,or P<0.001).Furthermore,AMPKα1 protein expression in the liver was significantly lower in Experimental feeding II group than in the Negative control group and Experimental feeding I group(P<0.001).These findings indicate that Stellera chamaejasme L.induces pathological damage to liver tissue in mice,inhibits the expression of glucose metabolism-related genes and proteins,and disrupts hepatic glucose metabolism.关键词
瑞香狼毒/小鼠/肝脏/损伤/糖代谢Key words
Stellera chamaejasme L./mice/liver/injury/glucose metabolism分类
畜牧业引用本文复制引用
周于凯,孙富燕,吕玺玮,何晓蝶,李鸿亮,李文鹏,刘道鑫,洪金,高磊..瑞香狼毒对小鼠肝脏损伤和糖代谢的影响[J].中国兽医杂志,2025,61(4):44-52,9.基金项目
国家自然科学基金地区项目(32260907) （32260907）
青海省科技厅自然科学基金青年项目(2022-ZJ-934Q) （2022-ZJ-934Q）
青海大学青年科研基金项目(2022-QNY-5) （2022-QNY-5）
青海大学省部共建三江源生态与高原农牧业国家重点实验室自主课题(2022-ZZ-09) （2022-ZZ-09）
