中国兽医杂志2025，Vol.61Issue(4)：62-69,8.DOI:10.20157/j.cnki.zgsyzz.2025.04.008
6种不同J亚群禽白血病病毒检测试剂盒的比较
Comparison of Six Test Kits for Detecting Avian Leukosis Virus Subgroup J
摘要
Abstract
In order to compare the detection results of six different avian leukosis virus subgroup J(ALV-J)detection kits for better avian leukosis purification and the assessment of chicken embryo cleanliness,this study first constructed a chick model carrying ALV-J by inoculating ALV-J reference strain into the yolk sac of specific pathogen free(SPF)chicken embryos.The embryos were infected and chick feces and anticoagulated blood were collected from the newly hatched chicks.The fecal filtrates and plasma were then inoculated into chicken embryo fibroblast DF-1 cells for virus culture.After 7 days of incubation,cell supernatants were collected and tested using enzyme-linked immunosorbent assay(ELISA)kits for ALV p27 antigen from companies A,B,and C,along with commercial magnetic microparticle chemiluminescence detection kits,colloidal gold test strips,and real-time fluorescence quantitative PCR kits.The samples tested included feces,different dilution samples of feces,virus isolation cell supernatants from feces,and plasma virus isolation cell supernatants.The results showed that the detection results from the six different kits for feces,fetal feces virus isolation cell supernatant,and plasma virus isolation cell supernatant were generally consistent,with the magnetic microparticle chemiluminescence detection kit offering the most intuitive readings.The positive and negative sample values were easily distinguishable.For the different dilution samples of feces,the A company's ALV p27 antigen ELISA kit,the magnetic microparticle chemiluminescence detection kit,and the real-time fluorescence quantitative PCR kit demonstrated relatively high sensitivity,capable of detecting low titer pathogens.These findings indicate that while the overall judgment of ALV-J detection using different methods did not show significant differences,the detection rates varied for low titer samples.Therefore,the method should be chosen based on the specific situation.关键词
J亚群禽白血病病毒(ALV-J)/p27抗原/酶联免疫吸附测定(ELISA)/磁微粒化学发光检测试剂盒/胶体金试纸条/实时荧光定量PCRKey words
avian leukosis virus subgroup J(ALV-J)/p27 antigen/enzyme-linked immunosorbent assay(ELISA)/magnetic microparticle chemiluminescence detection kit/colloidal gold test strip/real-time fluorescence quantitative PCR分类
畜牧业引用本文复制引用
赵方钰,崔慧珍,王一新,常爽,任志浩,崔文平,赵鹏..6种不同J亚群禽白血病病毒检测试剂盒的比较[J].中国兽医杂志,2025,61(4):62-69,8.基金项目
中央引导地方科技发展专项资金项目(YDZX2022127) （YDZX2022127）
