中国兽医杂志2025，Vol.61Issue(4)：104-112,9.DOI:10.20157/j.cnki.zgsyzz.2025.04.014
库页红景天黄酮组分对类风湿性关节炎的治疗作用及潜在分子机制
Therapeutic Effects and Potential Molecular Mechanisms of Flavonoid Components from Rhodiola sachalinensis A.Bor.on Rheumatoid Arthritis
摘要
Abstract
To investigate the in vitro antioxidant and anti-inflammatory effects,as well as the therapeutic potential and molecular mechanisms of Rhodiola sachalinensis flavonoid components(RSF)in rheumatoid arthritis(RA),this study utilized a previously established extraction and purification method to obtain high-purity RSF.The superoxide anion scavenging assay and ferric reducing antioxidant power(FRAP)assay were used to evaluate antioxidant capacity.An in vitro inflammation model was established using Raw264.7 murine macrophages.The anti-inflammatory effects of different RSF concentrations(1,2,and 4 mg/mL)were assessed by measuring the levels of inflammatory cytokines in the culture supernatant.Oxygen radical absorbance capacity(ORAC)assay was used to further assess antioxidant activity.An adjuvant-induced arthritis model was established in rats using complete Freund's adjuvant,and RSF was administered orally at different doses(2,4,and 6 mg/mL).The therapeutic effects and potential mechanisms were investigated by measuring ankle circumference,serum inflammatory cytokine levels,bone metabolism-related signaling factors,protein expression in the ankle joint,and histopathological changes.The results showed that the purified RSF exhibited strong superoxide anion scavenging ability and significantly enhanced FRAP antioxidant effects.RSF at different concentrations significantly inhibited the secretion of pro-inflammatory cytokines,including tumor necrosis factor-alpha(TNF-α),interleukin-1β(IL-1β),and prostaglandin-2(PEG2)in Raw264.7 cells(P<0.05 or P<0.01),while increasing ORAC values(P<0.05 or P<0.01).In animal experiments,the RA model was successfully established.Compared with the Model group,the Medium-dose RSF group exhibited a significant reduction in ankle swelling(P<0.01),as well as significantly lower serum levels of TNF-α,IL-1β,and PEG2(P<0.05 or P<0.01).The receptor activator of nuclear factor-κB ligand(RANKL)/osteoprotegerin(OPG)ratio was significantly reduced(P<0.05).Furthermore,protein expression levels of nuclear factor-kappaB(NF-κB)and RANKL in ankle joint tissues were significantly decreased(P<0.01),while OPG protein expression was significantly upregulated(P<0.01),leading to a marked reduction in the RANKL/OPG ratio(P<0.01).Additionally,arthritis index scores were significantly lower in the RSF-treated groups(P<0.05).These findings indicate that RSF exhibits strong antioxidant and anti-inflammatory effects in vitro and exerts therapeutic benefits in RA rats by reducing ankle swelling,lowering inflammatory cytokine levels,and improving ankle joint morphology.The underlying mechanism may involve the regulation of NF-κB and RANK-RANKL-OPG signaling pathways.关键词
类风湿性关节炎/库页红景天/黄酮组分/抗氧化作用/抗炎作用/分子机制Key words
rheumatoid arthritis/Rhodiola sachalinensis A.Bor./flavonoid component/antioxidant effect/anti-inflamma-tory effect/molecular mechanism分类
农业科技引用本文复制引用
王纯普,丁雪晴,李佳轩,张锦贝,潘欣,马莹慧,时念秋..库页红景天黄酮组分对类风湿性关节炎的治疗作用及潜在分子机制[J].中国兽医杂志,2025,61(4):104-112,9.基金项目
吉林省自然科学基金(YDZJ2023012YTS142) （YDZJ2023012YTS142）
