中国体外循环杂志2025，Vol.23Issue(2)：148-152,161,6.DOI:10.13498/j.cnki.chin.j.ecc.2025.02.12
离体心脏长时间在心肌保护液中低温保存对线粒体的影响
Effects of prolonged cold storage in cardioplegic solution on mitochondria in isolated hearts
摘要
Abstract
Objective To investigate the effect of cardioplegic solution(HTK)on isolated rat hearts during prolonged cold storage,observing changes in hemodynamics and cardiac tissue structure,and preliminarily explorring the effects of HTK on myocardial mitochondria under cold storage conditions.Methods 24 male SD rats weighing 280-360 g were randomly divided into three groups:sham group,HTK cold storage for 8 hours(HTK-8)and 12 hours(HTK-12).Cardiac hemodynamic changes were recorded using the Langendorff device,and structural changes in myocardial tissue were observed using HE staining,changes in cardiomyocyte mitochondria were assessed through transmission electron microscopy,and the expression of mitochondria-associated proteins was detected by Western blot.Results In terms of hemodynamics,the recovery rate of pump function and maximum end-diastolic pressure were significantly higher in HTK-8 than in HTK-12,with a statistically significant difference(P<0.05).HE staining showed that the cardiomyocyte structure of HTK-8 was relatively intact,and the degree of interstitial edema,cell swelling and fat changes were significantly less severe than that of HTK-12.Transmission electron microscopy showed mitochondrial swelling and cristae disruption in both HTK-8 and HTK-12 groups,but the changes were less severe in the HTK-8 group.In terms of mitochondrial function,the two proteins regulating mitochondrial fission and fusion changed significantly with the extension of preservation time,suggesting impaired mitochondrial function.Conclusion Prolonged cold storage of isolated hearts exacerbates mitochondrial structural and functional damage,contributing to overall cardiac function.关键词
心脏低温保存/HTK心肌保护液/心肌保护/线粒体/大鼠Key words
Prolonged cryopreservation/Histidine-Tryptophan-Ketoglutarate solution/Myocardial protection/Mitochondria/Rat引用本文复制引用
王一涵,李双磊,何潇一,肖苍松..离体心脏长时间在心肌保护液中低温保存对线粒体的影响[J].中国体外循环杂志,2025,23(2):148-152,161,6.基金项目
军委科技委基础加强计划重点研究项目(2019-JCJQ-ZD-195-00) （2019-JCJQ-ZD-195-00）
