中国体外循环杂志2025，Vol.23Issue(2)：162-169,8.DOI:10.13498/j.cnki.chin.j.ecc.2025.02.14
柠康酸对小鼠心肌缺血再灌注损伤的保护作用研究
Study on the protective effect of citraconate on myocardial ischemia-reperfusion injury in mice
摘要
Abstract
Objective To investigate the role of citraconate in LPS/IFN-γ induced inflammation of RAW264.7 macrophages and its effect on myocardial ischemia-reperfusion injury(MI/RI)in mice.Methods The inflammatory response of RAW264.7 macrophages was induced by LPS/IFN-γ,and the cell viability after citraconate treatment was evaluated by cell counting reagent(CCK-8).The inflammatory response of RAW264.7 macrophages was evaluated by real-time fluorescence quantitative PCR and western blot.The MI/RI model was constructed to compare the changes in cardiac function after intraperitoneal injection of exogenous citraconate in mice.The inflammatory response of the heart after MI/RI was determined by fluorescence immunostaining,real-time fluorescence quantitative PCR,and protein immunoblotting.The expression of BAX(Bcl-2 associated X)protein and apoptosis of myocardial cells after MI/RI were measured by Tunel.Results The dosage and treatment time of citraconate for LPS/IFN-γ-induced RAW264.7 macrophages were determined to be 1 mmol/L to 10 mmol/L for 4 hours;citraconate significantly alleviated LPS/IFN-γ induced inflammation in RAW264.7 macrophages and exhibited a dose-dependent effect.Citraconate treatment significantly reduced cardiac inflammation and myocardial cell apoptosis in mice after MI/RI,and significantly improved cardiac function in mice after MI/RI.Conclusion Citraconate improves the cardiac function of mice after MI/RI by alleviating cardiac inflammation and myocardial cell apoptosis.关键词
柠康酸/心肌缺血-再灌注损伤/RAW 264.7巨噬细胞/心脏功能/炎症反应/心肌细胞凋亡Key words
Citraconate/Myocardial ischemia-reperfusion injury/RAW 264.7 macrophages/Cardiac function/Inflammation/Myocardial cell apoptosis引用本文复制引用
封林奇,陈城,山文菊,蹇玉红,易蔚..柠康酸对小鼠心肌缺血再灌注损伤的保护作用研究[J].中国体外循环杂志,2025,23(2):162-169,8.基金项目
陕西省重点研发计划(2023-ZDLSF-39) （2023-ZDLSF-39）
