中国药科大学学报2025，Vol.56Issue(2)：148-154,7.DOI:10.11665/j.issn.1000-5048.2024050603
污水中26种痕量卡西酮类新精神活性物质的HPLC-MS/MS检测方法
Determination of 26 trace cathinones new psychoactive substances in sewage by HPLC-MS/MS
摘要
Abstract
A method for the pretreatment and qualitative detection of 26 trace cathinone new psychoactive substances in wastewater was established and applied in actual wastewater cases.The effluent samples were eluted on the Oasis PRiME HLB solid phase extraction column by ultra-pure water drenching and methanol solution,then dried with nitrogen at 40℃,and finally re-dissolved with 0.1%formic acid-acetonitrile solution(95∶5),and detected by liquid chromatography-tandem mass spectrometry,The effluent sample was determined by high-performance liquid chromatography-Tandem mass spectrometry(HPLC-MS/MS)using selected reaction monitoring(SRM)mode and separated on chromatographic column UPLC BEH C18(100 mm×2.1 mm,1.7μm)at 35℃with a mobile phase consisting of acetonitrile-0.1%formic acid in aqueous solution gradient elution.After methodological validation,the lower quantification of 26 cathinone new psychoactive substances could reach 1.50-3.00 ng/L.Among these,21 analytes fell within the concentration range of 1.50-375.0 ng/L,while 5 were detected in the range of 3.00-750.0 ng/L,the correlation coefficient was 0.99,within-and between-batch precision was less than 7.71%and 13.91%,respectively,and the extraction recoveries were higher than 92.64%.The method is simple,accurate,and sensitive,and can be used for cathinone detection and abuse monitoring.关键词
卡西酮/新精神活性物质/新型毒品分析/污水流行病学/超高效液相色谱-串联质谱Key words
cathinone/new psychoactive substances/new illegal drug analysis/wastewater-based epidemiology/ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)分类
药学引用本文复制引用
邓滨,朱娜,花镇东,王优美,苏梦翔..污水中26种痕量卡西酮类新精神活性物质的HPLC-MS/MS检测方法[J].中国药科大学学报,2025,56(2):148-154,7.基金项目
国家重点研发计划资助项目(2022YFC3300902) This study was supported by the National Key Research and Development Program of China(2022YFC3300902) （2022YFC3300902）
