中国药科大学学报2025，Vol.56Issue(2)：160-165,6.DOI:10.11665/j.issn.1000-5048.2024040302
暗紫贝母茎化学成分研究
Chemical constituents from the stems of Fritillaria unibracteata
摘要
Abstract
Chemical investigation of the stems of Fritillaria unibracteata P.K.Hsiao&K.C.Hsia resulted in the isolation of nine compounds,by means of silica gel column chromatography,and preparative HPLC.Based on spectroscopic and chemical evidence,these compounds were identified as:27-hydroxychlorogenone(1),sieboldogenin(2),(3β,25S)-spirost-5-ene-3,17,27-triol(3),laxogenin(4),tigogenone(5),cerevisterol(6),ergosterol peroxide(7),stigmaterol(8),and β-sitosterol(9).Compound 1 was a new compound,and compounds 2-9 were isolated from the stems of Fritillaria unibracteata for the first time.The inhibitory effects of compounds 1-9 on A549 cells were determined using the MTT method.The results show that compound 6 exhibits moderate inhibitory activity with an IC50 value of(14.16±1.11)μmol/L.关键词
暗紫贝母/茎/甾体/化学成分/细胞毒Key words
Fritillaria unibracteata/stem/steroid/chemical constituents/cytotoxicity分类
中医学引用本文复制引用
李敏,米雅慧,蒯海敏,胡晓龙,汪豪..暗紫贝母茎化学成分研究[J].中国药科大学学报,2025,56(2):160-165,6.基金项目
This study was supported by the Major Science and Technology Special Projects of Yunnan Province(202402AA310001) （202402AA310001）
the Expert Workstation Project of Yunnan Province(202305AF150096) （202305AF150096）
Diqing State-China Pharmaceutical University State-School Cooperation Project(2021ZXYD01) 云南省重大科技专项(202402AA310001) （2021ZXYD01）
云南省专家工作站项目(202305AF150096) （202305AF150096）
迪庆州-中国药科大学州校合作项目(2021ZXYD01) （2021ZXYD01）
