中国药科大学学报2025，Vol.56Issue(2)：166-175,10.DOI:10.11665/j.issn.1000-5048.2024090301
通络生骨胶囊原粉化学成分鉴定及牡荆苷含量测定
Identification of chemical components and determination of vitexin in the raw powder of Tongluo Shenggu capsule
吴革林 1范瑞鑫 2梁楚玲 3邢冷 4谢永建 4龚平 4周鹏 4李博3
作者信息
- 1. 浙江赞生药业有限公司,台州 318000||杭州赞生医药有限公司,杭州 310052
- 2. 中国药科大学(杭州)创新药物研究院,杭州 310018
- 3. 中国药科大学(杭州)创新药物研究院,杭州 310018||中国药科大学药学院,南京 211198
- 4. 浙江赞生药业有限公司,台州 318000
- 折叠
摘要
Abstract
The present study employed UPLC-MS/MS to analyze and identify compounds in the raw powder of Tongluo Shenggu capsules.An HPLC method for the determination of vitexin content was established.The analysis of this drug was performed on a 30℃thermostatic Acquity UPLC®BEH C18(2.1 mm×100 mm,1.7 μm)column,with the mobile phase comprising 0.2%formic acid-methanol flowing at 0.3 mL/min in a gradient elution manner.Mass spectrometry was detected by ESI sources in both positive and negative ion modes for qualitative identification of chemical constituents.12 flavonoid and 3 stilbenes compounds in the raw powder of Tongluo Shenggu capsules were successfully identified.Additionally,an HPLC method for the determination of vitexin content was established using a XBridge C18 column(4.6 mm×250 mm,5µm)with a mobile phase of 0.05%glacial acetic acid in methanol for gradient elution,at a column temperature of 30℃,a flow rate of 1.0 mL/min,and an injection volume of 20 μL.The method demonstrated good linearity in the concentration range of 10µg/mL to 40µg/mL(R=1.000)with an average recovery rate of 96.7%.The establishment of these methods provides a scientific basis for the quality control and development of the raw powder of Tongluo Shenggu capsules.关键词
通络生骨胶囊/化学成分/UPLC-MS/MS/牡荆苷/HPLCKey words
Tongluo Shenggucapsule/chemical components/UPLC-MS/MS/vitexin/HPLC分类
药学引用本文复制引用
吴革林,范瑞鑫,梁楚玲,邢冷,谢永建,龚平,周鹏,李博..通络生骨胶囊原粉化学成分鉴定及牡荆苷含量测定[J].中国药科大学学报,2025,56(2):166-175,10.
