中国药科大学学报2025，Vol.56Issue(2)：188-195,8.DOI:10.11665/j.issn.1000-5048.2024102701
耳聋左慈丸对HEI-OC1细胞氧化应激治疗作用的机制研究
Therapeutic mechanism of of Erlong Zuoci Pills on oxidative stress in HEI-OC1 cells
摘要
Abstract
To the present study aimed to investigate the protective effects of Erlong Zuoci Pills on oxidative stress induced by hydrogen peroxide(H2O2)in House Ear Institute-Organ of Corti 1(HEI-OC1)and to explore the mechanism by cellular metabolomics.There were 6 groups in the experiment:the control group,model group,three dose groups of ELZC(low,medium,and high),and positive control ascorbic acid group.The oxidative stress injury model was established in the HEI-OC1 by inducing 0.9 mmol/L H2O2 for 12 h.The proliferation of HEI-OC1 cells was observed by CCK-8 assay;the contents and activity of lactate hydrogenase(LDH),reactive oxygen species(ROS),and superoxide dismutase(SOD)in HEI-OC1 cells were detected by corresponding kits.Finally,the endogenous substances of cells were analyzed from the perspective of metabolomics.Compared with the model group,ELZC groups could significantly increase the cell proliferation rate after administration.Moreover,they could also ameliorate the increase of ROS and LDH content and the decrease of antioxidant enzyme SOD caused by H2O2.Metabolomic results revealed significant differences among multiple groups in the scores of partial least squares discriminant analysis.The ELZC group could relocate the model group back to the control group.The metabolic regulation of ELZC on oxidative stress in HEI-OC1 cells mainly affects nucleotide metabolism and amino acid metabolism.In summary,the results indicate that ELZC exhibits protective effects on H2O2-induced oxidative stress in HEI-OC1 cells.Additionally,this protective effect may be produced by increasing the content of amino acids such as uridine and phenylalanine,thereby regulating pathways such as pyrimidine metabolism,phenylalanine metabolism,biosynthesis of phenylalanine,tyrosine,and tryptophan,and histidine metabolism.关键词
耳聋左慈丸/小鼠耳蜗毛细胞株HEI-OC1/过氧化氢/氧化应激/代谢组学Key words
Erlong Zuoci Pills/HEI-OC1 cells/hydrogen peroxide/oxidative stress/metabolomics分类
药学引用本文复制引用
卢国侠,顾云双,郑亦文,彭英,阿基业,王广基,孙建国..耳聋左慈丸对HEI-OC1细胞氧化应激治疗作用的机制研究[J].中国药科大学学报,2025,56(2):188-195,8.基金项目
国家重点研发计划"政府间国际科技创新合作"重点专项资助项目(2017YFE0109600) This study was supported by the National Key Research and Development Program for Intergovernmental Cooperation in International Science and Technology Innovation(2017YFE0109600) （2017YFE0109600）
