中国药科大学学报2025，Vol.56Issue(2)：196-206,11.DOI:10.11665/j.issn.1000-5048.2024030502
不同阶段Gao-Binge模型中游离脂肪酸、三羧酸循环及酮体代谢谱研究
Plasma and hepatic free fatty acid,tricarboxylic acid cycle,and ketone bodies metabolic profiles in progressive Gao-Binge model
摘要
Abstract
To investigate the correlation between hepatic lipid accumulation and the metabolic profiles of free fatty acids(FFAs),tricarboxylic acid(TCA)cycle,and ketone body in alcoholic fatty liver disease(AFLD),a chronic plus acute alcohol feeding model(Gao-Binge model)was employed using C57BL/6N mice to simulate different stages of AFLD.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was employed to measure the levels of FFAs,TCA cycle intermediates,and ketone bodies in mouse liver tissue and plasma,followed by Pearson correlation analysis.The study revealed that both acute and chronic models showed significant increases in total FFAs,saturated FFAs and short-chain FFAs,as well as β-hydroxybutyric acid(HDBT)in plasma and liver,indicating FFA metabolic profile disturbances in the Gao-Binge model.Moreover,in both models,acetic acid(AA),2-Methylbutyric acid(2-meBA),and HDBT displayed strong positive correlations with hepatic injury markers in plasma and liver samples(for instance,in the acute model plasma data,r=0.834,0.699,0.818,P<0.05),while pyruvic acid(PRA)showed a strong negative correlation(r=-0.66,P<0.05).These findings suggest that FFAs,TCA cycle,and ketone body metabolism are disrupted in the alcoholic liver disease in mice model,and metabolites such as AA,2-meBA,HDBT and PRA may serve as potential biomarkers for AFLD,which would be helpful in the diagnosis and treatment of this disease.关键词
酒精性脂肪性肝病/脂肪酸/三羧酸循环/动物模型/Gao-Binge模型/相关性分析Key words
alcoholic fatty liver/fatty acids/tricarboxylic acid cycle/animal models/Gao-Binge model/correlation analysis/correlation analysis/Gao-Binge model分类
药学引用本文复制引用
黎安沁,庞陆鑫,柴媛媛,俞沁玮,江振洲,张陆勇..不同阶段Gao-Binge模型中游离脂肪酸、三羧酸循环及酮体代谢谱研究[J].中国药科大学学报,2025,56(2):196-206,11.基金项目
国家自然科学基金项目(No.81773827,No.82304642) This study was supported by the National Natural Science Foundation of China(No.81773827,No.82304642) （No.81773827,No.82304642）
